首页|Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm

Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
Background:Sand is often considered the preferred bedding material for dairy cows as it is thought to have lower bacterial counts than organic bedding materials and cows bedded on sand experience fewer cases of lameness and disease. Sand can also be efficiently recycled and reused, making it cost-effective. However, some studies have suggested that the residual organic material present in recycled sand can serve as a reservoir for commensal and pathogenic bacteria, although no studies have yet characterized the total bacterial community composition. Here we sought to characterize the bacterial community composition of a Wisconsin dairy farm bedding sand recycling system and its dynamics across several stages of the recycling process during both summer and winter using 16S rRNA gene amplicon sequencing. Results:Bacterial community compositions of the sand recycling system differed by both seasons and stage. Summer samples had higher richness and distinct community compositions, relative to winter samples. In both summer and winter samples, the diversity of recycled sand decreased with time drying in the recycling room. Compositionally, summer sand 14 d post-recycling was enriched in operational taxonomic units (OTUs) belonging to the genera Acinetobacter and Pseudomonas, relative to freshly washed sand and sand from cow pens. In contrast, no OTUs were found to be enriched in winter sand. The sand recycling system contained an overall core microbiota of 141 OTUs representing 68.45％± 10.33％SD of the total bacterial relative abundance at each sampled stage. The 4 most abundant genera in this core microbiota included Acinetobacter, Psychrobacter, Corynebacterium, and Pseudomonas. Acinetobacter was present in greater abundance in summer samples, whereas Psychrobacter and Corynebacterium had higher relative abundances in winter samples. Pseudomonas had consistent relative abundances across both seasons. Conclusions:These findings highlight the potential of recycled bedding sand as a bacterial reservoir that warrants further study.

BovineDairy farmMicrobiotaRecycled bedding sand16S rRNA sequencing

Hannah E.Pilch、Andrew J.Steinberger、Donald C.Sockett、Nicole Aulik、Garret Suen、Charles J.Czuprynski

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Department of Pathobiological Sciences,School of Veterinary Medicine,University of Wisconsin-Madison,Madison 53706,USA

Department of Bacteriology,University of Wisconsin-Madison,Madison 53706,USA

Microbiology Doctoral Training Program,University of Wisconsin-Madison,Madison 53706,USA

Wisconsin Veterinary Diagnostic Laboratory,School of Veterinary Medicine,University of Wisconsin-Madison,Madison 53706,USA

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Walter and Martha Renk Endowed Laboratory for Food Safety and the UW-Madison Food Research Institutea United States Department of Agriculture(USDA)National Institute of Food and Agriculture(NIFA)Food Safety Challenge Granta USDA NIFA Agricultural and Food Research Initiative Foundational Grant Foundation grant

20017–68003-265002020–67015-31576

2022

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCDCSCDSCI
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2022.13(2)
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