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畜牧与生物技术杂志(英文版)
中国科学技术协会
畜牧与生物技术杂志(英文版)

中国科学技术协会

李德发

季刊

1674-9782

zgxm@cau.edu.cn;xmzz@cau.edu.cn

010-62734403/2723

100193

中国农业大学西校区动物科技学院Journal of Animal Science and Biotechnology编辑部

畜牧与生物技术杂志(英文版)/Journal Journal of Animal Science and BiotechnologyCSCDCSTPCD北大核心SCI
查看更多>>定位为国内外公开发行的专业学术期刊，宗旨是立足国内，面向国际，为国内外畜牧科技工作者提供国际化学术共享的园地，促进我国与国际上其他国家在畜牧科学领域的交流；让中国畜牧科技走向世界，让世界了解中国畜牧科技。
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    Role of immunomodulatory probiotics in alleviating bacterial diarrhea in piglets:a systematic review

    Sudeb SahaFu NamaiKeita NishiyamaJulio Villena...
    2175-2199页
    查看更多>>摘要：Diarrhea is a common enteric disease in piglets that leads to high mortality and economic losses in swine produc-tion worldwide.Antibiotics are commonly used to prevent or treat diarrhea in piglets.However,irrational antibiotic use contributes to the development of resistance in bacteria and antibiotic residues in animal products,threatening public health,while causing gut microbiota dysbiosis and antibiotic-resistant bacterial infection in piglets.Therefore,the quest for alternative products(such as probiotics,prebiotics,organic acids,enzymes,essential oils,medium-chain fatty acids,zinc,and plant extracts)has recently been clearly emphasized through the increase in regulations regard-ing antibiotic use in livestock production.These antibiotic alternatives could lower the risk of antibiotic-resistant bacteria and meet consumer demand for antibiotic-free food.Several antibiotic alternatives have been proposed,including immunomodulatory probiotics,as candidates to reduce the need for antimicrobial therapy.Many studies have revealed that probiotics can avert and cure bacterial diarrhea by regulating the gut function and immune sys-tem of piglets.In this review,we focus on the major pathogenic bacteria causing piglet diarrhea,the research status of using probiotics to prevent and treat diarrhea,their possible mechanisms,and the safety issues related to the use of probiotics.Supplementation with probiotics is a possible alternative to antibiotics for the prevention or treatment of bacterial diarrhea in piglets.Furthermore,probiotics exert beneficial effects on feed efficiency and growth perfor-mance of piglets.Therefore,appropriate selection and strategies for the use of probiotics may have a positive effect on growth performance and also reduce diarrhea in piglets.This review provides useful information on probiotics for researchers,pig nutritionists,and the additive industry to support their use against bacterial diarrhea in piglets.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Advances in single-cell transcriptomics in animal research

    Yunan YanSenlin ZhuMinghui JiaXinyi Chen...
    2200-2215页
    查看更多>>摘要：Understanding biological mechanisms is fundamental for improving animal production and health to meet the grow-ing demand for high-quality protein.As an emerging biotechnology,single-cell transcriptomics has been gradually applied in diverse aspects of animal research,offering an effective method to study the gene expression of high-throughput single cells of different tissues/organs in animals.In an unprecedented manner,researchers have identi-fied cell types/subtypes and their marker genes,inferred cellular fate trajectories,and revealed cell-cell interactions in animals using single-cell transcriptomics.In this paper,we introduce the development of single-cell technology and review the processes,advancements,and applications of single-cell transcriptomics in animal research.We sum-marize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and health,reproductive performance,genetics,and disease models in different livestock species.Moreover,the prac-tical experience accumulated based on a large number of cases is highlighted to provide a reference for determin-ing key factors(e.g.,sample size,cell clustering,and cell type annotation)in single-cell transcriptomics analysis.We also discuss the limitations and outlook of single-cell transcriptomics in the current stage.This paper describes the comprehensive progress of single-cell transcriptomics in animal research,offering novel insights and sustainable advancements in agricultural productivity and animal health.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Improving the accuracy of genomic prediction in dairy cattle using the biologically annotated neural networks framework

    Xue WangShaolei ShiYousuf Ali KhanZhe Zhang...
    2216-2228页
    查看更多>>摘要：Background Biologically annotated neural networks(BANNs)are feedforward Bayesian neural network models that utilize partially connected architectures based on SNP-set annotations.As an interpretable neural network,BANNs model SNP and SNP-set effects in their input and hidden layers,respectively.Furthermore,the weights and connections of the network are regarded as random variables with prior distributions reflecting the manifestation of genetic effects at various genomic scales.However,its application in genomic prediction has yet to be explored.Results This study extended the BANNs framework to the area of genomic selection and explored the optimal SNP-set partitioning strategies by using dairy cattle datasets.The SNP-sets were partitioned based on two strategies-gene annotations and 100 kb windows,denoted as BANN_gene and BANN_100kb,respectively.The BANNs model was compared with GBLUP,random forest(RF),BayesB and BayesCn through five replicates of five-fold cross-valida-tion using genotypic and phenotypic data on milk production traits,type traits,and one health trait of 6,558,6,210 and 5,962 Chinese Holsteins,respectively.Results showed that the BANNs framework achieves higher genomic pre-diction accuracy compared to GBLUP,RF and Bayesian methods.Specifically,the BANN_1 00kb demonstrated superior accuracy and the BANN_gene exhibited generally suboptimal accuracy compared to GBLUP,RF,BayesB and BayesCπacross all traits.The average accuracy improvements of BANN_100kb over GBLUP,RF,BayesB and BayesCπ were 4.86％,3.95％,3.84％and 1.92％,and the accuracy of BANN_gene was improved by 3.75％,2.86％,2.73％and 0.85％compared to GBLUP,RF,BayesB and BayesCπ,respectively across all seven traits.Meanwhile,both BANN_100kb and BANN_gene yielded lower overall mean square error values than GBLUP,RF and Bayesian methods.Conclusion Our findings demonstrated that the BANNs framework performed better than traditional genomic pre-diction methods in our tested scenarios,and might serve as a promising alternative approach for genomic prediction in dairy cattle.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Combining genetic markers,on-farm information and infrared data for the in-line prediction of blood biomarkers of metabolic disorders in Holstein cattle

    Lucio F.M.MotaDiana GiannuzziSara PegoloHugo Toledo-Alvarado...
    2229-2241页
    查看更多>>摘要：Background Various blood metabolites are known to be useful indicators of health status in dairy cattle,but their routine assessment is time-consuming,expensive,and stressful for the cows at the herd level.Thus,we evaluated the effectiveness of combining in-line near infrared(NIR)milk spectra with on-farm(days in milk[DIM]and parity)and genetic markers for predicting blood metabolites in Holstein cattle.Data were obtained from 388 Holstein cows from a farm with an AfiLab system.NIR spectra,on-farm information,and single nucleotide polymorphisms(SNP)markers were blended to develop calibration equations for blood metabolites using the elastic net(ENet)approach,considering 3 models:(1)Model 1(M1)including only NIR information,(2)Model 2(M2)with both NIR and on-farm information,and(3)Model 3(M3)combining NIR,on-farm and genomic information.Dimension reduction was con-sidered for M3 by preselecting SNP markers from genome-wide association study(GWAS)results.Results Results indicate that M2 improved the predictive ability by an average of 19％for energy-related metabolites(glucose,cholesterol,NEFA,BHB,urea,and creatinine),20％for liver function/hepatic damage,7％for inflammation/innate immunity,24％for oxidative stress metabolites,and 23％for minerals compared to M1.Meanwhile,M3 further enhanced the predictive ability by 34％for energy-related metabolites,32％for liver function/hepatic damage,22％for inflammation/innate immunity,42.1％for oxidative stress metabolites,and 41％for minerals,compared to M1.We found improved predictive ability of M3 using selected SNP markers from GWAS results using a threshold of＞2.0 by 5％for energy-related metabolites,9％for liver function/hepatic damage,8％for inflammation/innate immunity,22％for oxidative stress metabolites,and 9％for minerals.Slight reductions were observed for phosphorus(2％),ferric-reducing antioxidant power(1％),and glucose(3％).Furthermore,it was found that prediction accuracies are influenced by using more restrictive thresholds(-log10(P-value)＞2.5 and 3.0),with a lower increase in the predictive ability.Conclusion Our results highlighted the potential of combining several sources of information,such as genetic mark-ers,on-farm information,and in-line NIR infrared data improves the predictive ability of blood metabolites in dairy cattle,representing an effective strategy for large-scale in-line health monitoring in commercial herds.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Host genetics and gut microbiota synergistically regulate feed utilization in egg-type chickens

    Wenxin ZhangFangren LanQianqian ZhouShuang Gu...
    2242-2261页
    查看更多>>摘要：Background Feed efficiency is a crucial economic trait in poultry industry.Both host genetics and gut microbiota influence feed efficiency.However,the associations between gut microbiota and host genetics,as well as their com-bined contributions to feed efficiency in laying hens during the late laying period,remain largely unclear.Methods In total,686 laying hens were used for whole-genome resequencing and liver transcriptome sequencing.16S rRNA gene sequencing was conducted on gut chyme(duodenum,jejunum,ileum,and cecum)and fecal samples from 705 individuals.Bioinformatic analysis was performed by integrating the genome,transcriptome,and microbi-ome to screen for key genetic variations,genes,and gut microbiota associated with feed efficiency.Results The heritability of feed conversion ratio(FCR)and residual feed intake(RFI)was determined to be 0.28 and 0.48,respectively.The ileal and fecal microbiota accounted for 15％and 10％of the FCR variance,while the jeju-nal,cecal,and fecal microbiota accounted for 20％,11％,and 10％of the RFI variance.Through SMR analysis based on summary data from liver eQTL mapping and GWAS,we further identified four protein-coding genes,SUCLA2,TNFSF13B,SERTM1,and MARVELD3,that influence feed efficiency in laying hens.The SUCLA2 and TNFSF13B genes were significantly associated with SNP 1:25664581 and SNP rs312433097,respectively.SERTM1 showed significant associa-tions with rs730958360 and 1:33542680 and is a potential causal gene associated with the abundance of Corynebac-teriaceae in feces.MARVELD3 was significantly associated with the 1:135348198 and was significantly correlated with the abundance of Enterococcus in ileum.Specifically,a lower abundance of Enterococcus in ileum and a higher abundance of Corynebacteriaceae in feces were associated with better feed efficiency.Conclusions This study confirms that both host genetics and gut microbiota can drive variations in feed efficiency.A small portion of the gut microbiota often interacts with host genes,collectively enhancing feed efficiency.There-fore,targeting both the gut microbiota and host genetic variation by supporting more efficient taxa and selective breeding could improve feed efficiency in laying hens during the late laying period.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Progesterone regulates tissue non-specific alkaline phosphatase(TNSALP)expression and activity in ovine utero-placental tissues

    Claire StenhouseKatherine M.HalloranEmily C.HoskinsRobyn M.Moses...
    2262-2275页
    查看更多>>摘要：Background Tissue non-specific alkaline phosphatase(TNSALP;encoded by the ALPL gene)has a critical role in the postnatal regulation of phosphate homeostasis,yet howTNSALP activity and expression are regulated during pregnancy remain largely unknown.This study tested the hypothesis that progesterone(P4)and/or interferon tau(IFNT)regulate TNSALP activity during pregnancy in sheep.Methods In Exp.1,ewes were bred and received daily intramuscular injections of either corn oil vehicle(CO)or 25 mg progesterone in CO(P4)for the first 8 days of pregnancy and were hysterectomized on either Day 9,12,or 125 of gestation.In Exp.2,ewes were fitted with intrauterine catheters on Day 7 of the estrous cycle and received daily intramuscular injections of 50 mg P4 in CO and/or 75 mg progesterone receptor antagonist(RU486)in CO from Days 8 to 15,and twice daily intrauterine injections of either control proteins(CX)or IFNT(25 μg/uterine horn/d)from Days 11 to 15(treatment groups:P4+CX;P4+IFNT;RU486+P4+CX;and RU486+P4+IFNT)and were hysterec-tomized on Day 16.Results In Exp.1,endometria from ewes administered P4 had greater expression of ALPL mRNA than ewes adminis-tered CO on Day 12.TNSALP activity appeared greater in the epithelia,stratum compactum stroma,and endothelium of the blood vessels in the endometrium and myometrium from ewes administered P4 than ewes administered CO on Day 12.On Day 125,TNSALP activity localized to uterine epithelial and endothelial cells,independent of P4 treat-ment.TNSALP activity in placentomes appeared greater in P4 treated ewes and was detected in endothelial cells and caruncular tissue in P4 treated but not CO treated ewes.In Exp.2,endometrial homogenates from ewes admin-istered RU486+P4+CX had lowerTNSALP activity those for P4+CX and P4+IFNT ewes.ImmunoreactiveTNSALP protein appeared greater in the mid-and deep-glandular epithelia in RU486+P4+CX treated ewes as compared to the other treatment groups.Enzymatic activity appeared greater on the apical surface of the deep glandular epi-thelia in endometria from ewes treated with RU486+P4+CX compared to the other treatment groups.Conclusions These results suggest that P4,but not IFNT,regulates the expression and activity of TNSALP in utero-placental tissues and has the potential to contribute to the regulation of phosphate availability that is critical for con-ceptus development during pregnancy.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    From follicle to blastocyst:microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality

    Camilla BenedettiKrishna Chaitanya PavaniYannick GansemansNima Azari-Dolatabad...
    2276-2292页
    查看更多>>摘要：Background Within the follicular fluid,extracellular vesicles(EVs)guide oocyte growth through their cargo micro-RNAs(miRNAs).Here,we investigated the role of EVs and their cargo miRNAs by linking the miRNAs found in EVs,derived from the fluid of an individual follicle,to the ability of its oocyte to become a blastocyst(competent)or not(non-competent).Methods Bovine antral follicles were dissected,categorized as small(2-4 mm)or large(5-8 mm)and the corre-sponding oocytes were subjected to individual maturation,fertilization and embryo culture to the blastocyst stage.Follicular fluid was pooled in 4 groups(4 replicates)based on follicle size and competence of the corresponding oocyte to produce a blastocyst.Follicular fluid-derived EVs were isolated,characterized,and subjected to miRNA-sequencing(Illumina Miseq)to assess differential expression(DE)in the 4 groups.Functional validation of the effect of miR-34c on embryo development was performed by supplementation of mimics and inhibitors during in vitro maturation(IVM).Results We identified 16 DE miRNAs linked to oocyte competence when follicular size was not considered.Within the large and small follicles,46 DE miRNAs were driving blastocyst formation in each group.Comparison of EVs from competent small and large follicles revealed 90 DE miRNAs.Cell regulation,cell differentiation,cell cycle,and metabolic process regulation were the most enriched pathways targeted by the DE miRNAs from competent oocytes.We identified bta-miR-34c as the most abundant in follicular fluid containing competent oocytes.Supple-mentation of miR-34c mimic and inhibitor during IVM did not affect embryo development.However,blastocyst qual-ity,as evidenced by higher cell numbers,was significantly improved following oocyte IVM in the presence of miR-34c mimics,while miR-34c inhibitors resulted in the opposite effect.Conclusion This study demonstrates the regulatory effect of miRNAs from follicular fluid-derived EVs on oocyte competence acquisition,providing a further basis for understanding the significance of miRNAs in oocyte maturation and embryonic development.Up-regulation of miR-34c in EVs from follicular fluid containing competent oocytes and the positive impact of miR-34c mimics added during IVM on the resulting blastocysts indicate its pivotal role in oocyte competence.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Visfatin(NAMPT)affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle

    Kamil DobrzynGrzegorz KopijMarta KiezunEwa Zaobidna...
    2293-2315页
    查看更多>>摘要：Background The pituitary belongs to the most important endocrine glands involved in regulating reproductive functions.The proper functioning of this gland ensures the undisturbed course of the oestrous cycle and affects the female's reproductive potential.It is believed that visfatin,a hormone belonging to the adipokine family,may regulate reproductive functions in response to the female's metabolic state.Herein we verified the hypothesis that suggests a modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle.Results RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 202 genes(95 up-regulated and 107 down-regulated in the presence of visfatin,when compared to the non-treated controls),assigned to 318 gene ontology terms.We revealed changes in the frequency of alternative splicing events(235 cases),as well as long noncoding RNA expression(79 cases)in the presence of the adipokine.The identified genes were associated,among others,with reproductive system development,epithelial cell proliferation,positive regulation of cell development,gland morphogenesis and cell chemotaxis.Conclusions The obtained results indicate a modulatory influence of visfatin on the regulation of the porcine tran-scriptome and,in consequence,pituitary physiology during the mid-luteal phase of the oestrous cycle.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
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    Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation

    Yijing HeTian hang YuHeran LiQinfeng Sun...
    2316-2332页
    查看更多>>摘要：Background Polystyrene nanoplastics(PS-NPs)are becoming increasingly prevalent in the environment with great advancements in plastic products,and their potential health hazard to animals has received much attention.Several studies have reported the toxicity of PS-NPs to various tissues and cells;however,there is a paucity of information about whether PS-NPs exposure can have toxic effects on mammalian oocytes,especially livestock.Herein,porcine oocytes were used as the model to investigate the potential effects of PS-NPs on mammalian oocytes.Results The findings showed that different concentrations of PS-NPs(0,25,50 and 100 μg/mL)entering into porcine oocytes could induce mitochondrial stress,including a significant decrease in mitochondrial membrane potential(MMP),and the destruction of the balance of mitochondrial dynamic and micromorphology.Furthermore,there was a marked increase in reactive oxygen species(ROS),which led to oocyte lipid peroxidation(LPO).PS-NPs expo-sure induced abnormal intracellular iron overload,and subsequently increased the expression of transferrin receptor(TfRC),solute carrier family 7 member 11(SLC7a11),and acyl-CoA synthetase long-chain family member 4(ACSL4),which resulted in ferroptosis in oocytes.PS-NPs also induced oocyte maturation failure,cytoskeletal dysfunction and DNA damage.Cotreatment with 5 μmol/L ferrostatin-1(Fer-1,an inhibitor of ferroptosis)alleviated the cellular toxicity associated with PS-NPs exposure during porcine oocyte maturation.Conclusions In conclusion,PS-NPs caused ferroptosis in porcine oocytes by increasing oxidative stress and altering lipid metabolism,leading to the failure of oocyte maturation.
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    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified Mil oocytes potentially by promoting histone lactylation

    Yaozong WeiBo PanJianpeng QinBeijia Cao...
    2333-2350页
    查看更多>>摘要：Background Previous studies have shown that the vitrification of metaphase Ⅱ(MⅡ)oocytes significantly represses their developmental potential.Abnormally increased oxidative stress is the probable factor;however,the underlying mechanism remains unclear.The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate.Accumulating evidences implied that TW-7 was a powerful antioxidant,while its prospective application in oocyte cryopreservation has not been reported.Result Here,we found that parthenogenetic activation(PA)zygotes derived from vitrified MⅡ oocytes showed elevated ROS level and delayed progression of pronucleus formation.Addition of 25 μmol/LTW-7 in warming,recov-ery,PA,and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MⅡ oocytes,furtherly increase proteins related to histone lactylation such as LDHA,LDHB,and EP300 and finally improve histone lactylation in PA zygotes.The elevated histone lactylation facilitated the expression of minor zygotic genome activa-tion(ZGA)genes and preimplantation embryo development.Conclusions Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse Mil oocytes and found a potent and easy-obtained short peptide that could sig-nificantly rescue the decreased developmental potential of vitrified oocytes,which would potentially contribute to reproductive medicine,animal protection,and breeding.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据