首页|Imprinting at the KBTBD6 locus involves species-specific maternal methylation and monoallelic expression in livestock animals

Imprinting at the KBTBD6 locus involves species-specific maternal methylation and monoallelic expression in livestock animals

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Background The primary differentially methylated regions(DMRs)which are maternally hypermethylated serve as imprinting control regions(ICRs)that drive monoallelic gene expression,and these ICRs have been investigated due to their implications in mammalian development.Although a subset of genes has been identified as imprinted,in-depth comparative approach needs to be developed for identification of species-specific imprinted genes.Here,we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of imprinting.Results Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos,we identified a maternally hypermethylated DMR between the embryos at the KBTBD6 promoter CpG island and paternal monoallelic expression of KBTBD6.Also,in analyzed domesticated mammals but not in humans,non-human primates and mice,the KBTBD6 promoter CpG islands were methylated in oocytes and/or allelically methyl-ated in tissues,and monoallelic KBTBD6 expression was observed,indicating livestock-specific imprinting.Further analysis revealed that these CpG islands were embedded within transcripts in porcine and bovine oocytes which coexisted with an active transcription mark and DNA methylation,implying the presence of transcription-dependent imprinting.Conclusions In this study,our comparative approach revealed an imprinted expression of the KBTBD6 gene in domesticated mammals,but not in humans,non-human primates,and mice which implicates species-specific evolution of genomic imprinting.

Differentially methylated regionDomesticated mammalImprintingKBTBD6Parthenogenetic

Jinsoo Ahn、In-Sul Hwang、Mi-Ryung Park、Seongsoo Hwang、Kichoon Lee

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Department of Animal Sciences,The Ohio State University,Columbus,OH 43210,USA

Animal Biotechnology Division,Rural Development Administration,National Institute of Animal Science,Jeonbuk 55365,Republic of Korea

Columbia Center for Translational Immunology,Columbia University Irving Medical Center,Columbia University,New York,NY 10032,USA

Animal Welfare Research Team,National Institute of Animal Science,RDA,1500,Kongjwipatjwi-ro,Jeollabuk-do 55365,Republic of Korea

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United States Department of Agriculture National Institute of Food and Agriculture Hatch Grant

OHO01304

2024

10.1186/s40104-023-00931-3

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(1)
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