Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

Estel Vi?olas-Vergés ¹Jordi Ribas-Maynou ¹Isabel Barranco ²Camila Peres Rubio ²Sergi Bonet ¹Jordi Roca ²Marc Yeste³

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作者信息

1. Unit of Cell Biology,Department of Biology,Faculty of Sciences,University of Girona,Girona,Spain;Biotechnology of Animal and Human Reproduc-tion(TechnoSperm),Institute of Food and Agricultural Technology,University of Girona,Girona,Spain
2. Department of Medicine and Animal Surgery,Faculty ofVeterinary Science,University of Murcia,Murcia,Spain
3. Unit of Cell Biology,Department of Biology,Faculty of Sciences,University of Girona,Girona,Spain;Biotechnology of Animal and Human Reproduc-tion(TechnoSperm),Institute of Food and Agricultural Technology,University of Girona,Girona,Spain;Catalan Institution for Research and Advanced Studies(ICREA),S08010 Barcelona,Spain
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Abstract

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development.In some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF one.Yet,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been interrogated.Results This study determined chromatin protamination(Chromomycin A3 test,CMA3),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 mL of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P＞0.05),chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P＞0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;P＜0.01);this index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;P＜0.001).Conclusion While sperm DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the PSRF.This could be related to the OSi of each fraction.

Key words

Chromatin/Condensation/DNA integrity/Ejaculate fractions/Pig/Protamination/Sperm

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基金项目

European Union's Horizon 2020 research and innovation scheme under the Marie Sk?odowska-Curie(801342)

Tecniospring INDUSTRY(TECSPR-19-1-0003)

Ministry of Science and Innovation,Spain(PID2020-113320RB-I00)

Ministry of Science and Innovation,Spain(PID2020-113493RB-I00)

Ministry of Science and Innovation,Spain(RYC2021-034546-Ⅰ)

Ministry of Science and Innovation,Spain(RYC2021-034764-Ⅰ)

Catalan Agency for Management of University and Research Grants()

Regional Government of Catalonia,Spain(2017-SGR-1229)

Regional Government of Catalonia,Spain(2021-SGR-00900)

Seneca Foundation,Regional Government of Murcia,Spain(21935/PI/22)

La Marató deTV3 Foundation(214/857-202039)

Catalan Institution for Research and Advanced Studies(ICREA)()

出版年

2024

畜牧与生物技术杂志(英文版)

中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD

影响因子：0.765

ISSN：1674-9782

参考文献量63

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