Rosemary extract improves egg quality by altering gut barrier function,intestinal microbiota and oviductal gene expressions in late-phase laying hens

Lianhua Zhang ¹Junwei Ge ²Fei Gao ³Min Yang ²Hui Li ¹Fei Xia ¹Hongtong Bai ¹Xiangshu Piao ⁴Zhiying Sun ⁵Lei Shi¹

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作者信息

1. Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;China National Botanical Garden,Bei-jing 100093,China
2. Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;China National Botanical Garden,Bei-jing 100093,China;College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
3. Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;China National Botanical Garden,Bei-jing 100093,China;University of Chinese Academy of Sciences,Beijing 100049,China
4. State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China
5. College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
折叠

Abstract

Background Rosemary extract(RE)has been reported to exert antioxidant property.However,the application of RE in late-phase laying hens on egg quality,intestinal barrier and microbiota,and oviductal function has not been systematically studied.This study was investigated to detect the potential effects of RE on performance,egg quality,serum parameters,intestinal heath,cecal microbiota and metabolism,and oviductal gene expressions in late-phase laying hens.A total of 210 65-week-old"JingTint 6"laying hens were randomly allocated into five treatments with six replicates and seven birds per replicate and fed basal diet(CON)or basal diet supplemented with chlortetracycline at 50 mg/kg(CTC)or RE at 50 mg/kg(RE50),100 mg/kg(RE100),and 200 mg/kg(RE200).Results Our results showed that RE200 improved(P＜0.05)Haugh unit and n-6/n-3 of egg yolk,serum superoxide dismutase(SOD)compared with CON.No significant differences were observed for Haugh unit and n-6/n-3 of egg yolk among CTC,RE50,RE100 and RE200 groups.Compared with CTC and RE50 groups,RE200 increased serum SOD activity on d 28 and 56.Compared with CON,RE supplementation decreased(P＜0.05)total cholesterol(TC)level.CTC,RE100 and RE200 decreased(P＜0.05)serum interleukin-6(IL-6)content compared with CON.CTC and RE200 increased jejunal mRNA expression ofZO-1 and Occludin compared with CON.The biomarkers of cecal microbiota and metabolite induced by RE 200,including Firmicutes,Eisenbergiella,Paraprevotella,Papillibacter,and butyrate,were closely associated with Haugh unit,n-6/n-3,SOD,IL-6,and TC.PICRUSt2 analysis indicated that RE altered carbohydrate and amino acid metabolism of cecal microbiota and increased butyrate synthesizing enzymes,includ-ing 3-oxoacid CoA-transferase and butyrate-acetoacetate CoA-transferase.Moreover,transcriptomic analysis revealed that RE200 improved gene expressions and functional pathways related to immunity and albumen formation in the oviductal magnum.Conclusions Dietary supplementation with 200 mg/kg RE could increase egg quality of late-phase laying hens via modulating intestinal barrier,cecal microbiota and metabolism,and oviductal function.Overall,RE could be used as a promising feed additive to improve egg quality of laying hens at late stage of production.

Key words

Intestinal health/Laying hens/Microbiota/Oviductal function/Phytochemicals/Production

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基金项目

China Postdoctoral Science Foundation(2022M723370)

Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23080603)

出版年

2024

畜牧与生物技术杂志(英文版)

中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD

影响因子：0.765

ISSN：1674-9782

参考文献量54

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