首页|Enhancement of porcine in vitro embryonic development through luteolin-mediated activation of the Nrf2/Keap1 signaling pathway

Enhancement of porcine in vitro embryonic development through luteolin-mediated activation of the Nrf2/Keap1 signaling pathway

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Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxy-gen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Luteolin(Lut)has been documented for its protective effects against oxidative stress in various studies.However,its specific role in embryonic development remains unexplored.This study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying mechanism.Results After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5 μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the control.Measurements on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the control.Moreover,Lut supplementation significantly augmented mitochondrial content and membrane potential.Intriguingly,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription levels.To further validate the relation-ship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of Lut.The positive effects of Lut on developmental competence were negated by brusatol treatment.Conclusions Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contrib-utes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.

LuteolinMitochondrial functionNrf2/Keap1 signaling pathwayOxidative stressPorcine embryo development

Se-Been Jeon、Pil-Soo Jeong、Min Ju Kim、Hyo-Gu Kang、Bong-Seok Song、Sun-Uk Kim、Seong-Keun Cho、Bo-Woong Sim

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Futuristic Animal Resource & Research Center(FARRC),Korea Research Institute of Bioscience and Biotechnology(KRIBB),Cheongju 28116,Republic of Korea

Department of Animal Science,College of Natural Resources & Life Science,Pusan National University,Miryang 50463,Republic of Korea

Depart-ment of Animal Science and Biotechnology,College of Agriculture and Life Science,Chungnam National University,Daejeon 34134,Republic of Korea

Department of Functional Genomics,University of Science and Technol-ogy,Daejeon 34113,Republic of Korea

Department of Animal Science,Life and Industry Convergence Research Institute(RICRI),College of Natural Resources & Life Science,Pusan National University,Miryang 50463,Republic of Korea

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Korea Research Institute of Bioscience and Biotechnology(KRIBB)Research Initiative Program,Republic of Korea,Republic of KorKorea Research Institute of Bioscience and Biotechnology(KRIBB)Research Initiative Program,Republic of Korea,Republic of Kor

KGM4252331KGM5382322

2024

10.1186/s40104-023-00947-9

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(2)
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