Composition,function,and timing:exploring the early-life gut microbiota in piglets for probiotic interventions

Jianping Quan ¹Cineng Xu ²Donglin Ruan ²Yong Ye ²Yibin Qiu ²Jie Wu ²Shenping Zhou ²Menghao Luan ²Xiang Zhao ²Yue Chen ²Danyang Lin ²Ying Sun ²Jifei Yang ²Enqin Zheng ³Gengyuan Cai ⁴Zhenfang Wu ¹Jie Yang³

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作者信息

1. College of Animal Science and National Engineering Research Center for Breeding Swine Industry,South China Agricultural University,Guangzhou,Guangdong,People's Republic of China;Guangdong Provincial Key Labora-tory of Agro-Animal Genomics and Molecular Breeding,South China Agricul-tural University,Guangzhou,Guangdong,China;Yunfu Subcenter of Guang-dong Laboratory for Lingnan Modern Agriculture,Yunfu,Guangdong,China;National Engineering Research Center for Breeding Swine Industry,Wens Foodstuff Group Co.,Ltd
2. College of Animal Science and National Engineering Research Center for Breeding Swine Industry,South China Agricultural University,Guangzhou,Guangdong,People's Republic of China
3. College of Animal Science and National Engineering Research Center for Breeding Swine Industry,South China Agricultural University,Guangzhou,Guangdong,People's Republic of China;Guangdong Provincial Key Labora-tory of Agro-Animal Genomics and Molecular Breeding,South China Agricul-tural University,Guangzhou,Guangdong,China
4. College of Animal Science and National Engineering Research Center for Breeding Swine Industry,South China Agricultural University,Guangzhou,Guangdong,People's Republic of China;Guangdong Provincial Key Labora-tory of Agro-Animal Genomics and Molecular Breeding,South China Agricul-tural University,Guangzhou,Guangdong,China;Yunfu Subcenter of Guang-dong Laboratory for Lingnan Modern Agriculture,Yunfu,Guangdong,China
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Abstract

Background The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous effects.However,the optimal timeframe for introducing probiotics to achieve this outcome remains uncertain.Results In the context of this investigation,we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time points.Simultaneously,we gathered vaginal and fecal samples from 23 sows.Employing 16S rRNA gene and metagenomic sequencing methodologies,we conducted a comprehensive analysis of the fluctuation patterns in microbial composition,functional capacity,interaction networks,and colo-nization resistance within the gut microbiota of piglets.As the piglets progressed in age,discernible modifications in intestinal microbial diversity,composition,and function were observed.A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal piglets.By D21,the microbial interaction network displayed a more concise and efficient configuration,accompa-nied by enhanced colonization resistance relative to the other two time points.Moreover,we identified three strains of Ruminococcus sp.at D10 as potential candidates for improving piglets'weight gain during the weaning phase.Conclusions The findings of this study propose that D10 represents the most opportune juncture for the introduc-tion of external probiotic interventions during the early stages of piglet development.This investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forth-coming probiotic interventions.

Key words

Colonization resistance/Intestinal microbiota/Piglet/Weaning/Weight gain

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基金项目

Key Technologies Research and Development Program of Guangdong Province project(2022B0202090002)

China Postdoctoral science Foundation(2021M701263)

Local Innovative and Research Teams Project of Guangdong Province(2019BT02N630)

Project of Swine Innovation Team in the Guangdong Modern Agricultural Research System(2023KJ126)

出版年

2024

畜牧与生物技术杂志(英文版)

中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD

影响因子：0.765

ISSN：1674-9782

参考文献量67

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