首页|Gut microbiota intervention attenuates thermogenesis in broilers exposed to high temperature through modulation of the hypothalamic 5-HT pathway
Gut microbiota intervention attenuates thermogenesis in broilers exposed to high temperature through modulation of the hypothalamic 5-HT pathway
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国家科技期刊平台
NETL
NSTL
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Background Broilers have a robust metabolism and high body temperature,which make them less tolerant to high-temperature(HT)environments and more susceptible to challenges from elevated temperatures.Gut microbes,functioning as symbionts within the host,possess the capacity to significantly regulate the physiological functions and environmental adaptability of the host.This study aims to investigate the effects of gut microbial intervention on the body temperature and thermogenesis of broilers at different ambient temperatures,as well as the underlying mechanism involving the"gut-brain"axis.Methods Broilers were subjected to gut microbiota interference with or without antibiotics(control or ABX)starting at 1 day of age.At 21 day of age,they were divided into 4 groups and exposed to different environments for 7 d:The control and ABX groups at room temperature(RT,24±1 ℃,60％ relative humidity(RH),24 h/d)and the control-HT and ABX-HT groups at high temperature(HT,32±1 ℃,60％RH,24 h/d).Results The results demonstrated that the antibiotic-induced gut microbiota intervention increased body weight and improved feed conversion in broiler chickens(P＜0.05).Under HT conditions,the microbiota intervention reduced the rectal temperature of broiler chickens(P＜0.05),inhibited the expression of avUCP and thermogenesis-related genes in breast muscle and liver(P＜0.05),and thus decreased thermogenesis capacity.Furthermore,the gut microbiota intervention blunted the hypothalamic-pituitary-adrenal axis and hypothalamic-pituitary-thyroid axis activation induced by HT conditions.By analyzing the cecal microbiota composition of control and ABX chickens maintained under HT conditions,we found that Alistipes was enriched in control chickens.In contrast,antibiotic-induced gut microbiota intervention resulted in a decrease in the relative abundance of Alistipes(P＜0.05).Moreover,this difference was accompanied by increased hypothalamic 5-hydroxytryptamine(5-HT)content and TPH2 expres-sion(P＜0.05).Conclusions These findings underscore the critical role of the gut microbiota in regulating broiler thermogenesis via the gut-brain axis and suggest that the hypothalamic 5-HT pathway may be a potential mechanism by which the gut microbiota affects thermoregulation in broilers.
Sheng Li、Xiaoqing Li、Kai Wang、Yansen Li、Kentaro Nagaoka、Chunmei Li
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Research Centre for Livestock Environmental Control and Smart Production,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
Laboratory of Veterinary Physiology,Department of Veterinary Medicine,Faculty of Agriculture,Tokyo University of Agriculture and Technology,Tokyo 183-8509,Japan
国家科技期刊平台
NSTL
万方数据
