Abstract
Background This work aimed to investigate the potential benefits of administering Prevotella and its primary metabolite succinate on performance,hepatic lipid accumulation and gut microbiota in laying hens.Results One hundred and fifty 58-week-old Hyline Brown laying hens,with laying rate below 80％ and plasma tri-glyceride(TG)exceeding 5 mmol/L,were used in this study.The hens were randomly allocated into 5 groups and sub-jected to one of the following treatments:fed with a basal diet(negative control,NC),oral gavage of 3 mL/hen saline every other day(positive control,PC),gavage of 3 mL/hen Prevotella melaninogenica(107 CFU/mL,PM)or 3 mL/hen Prevotella copri(107 CFU/mL,P.copri)every other day,and basal diet supplemented with 0.25％ sodium succinate(Succinate).The results showed that PM and P.copri treatments significantly improved laying rate compared to the PC(P＜0.05).The amount of lipid droplet was notably decreased by PM,P.copri,and Succinate treatments at week 4 and decreased by P.copri at week 8(P＜0.05).Correspondingly,the plasma TG level in Succinate group was lower than that of PC(P＜0.05).Hepatic TG content,however,was not significantly influenced at week 4 and 8(P＞0.05).PM treatment increased(P＜0.05)the mRNA levels of genes PGC-1β and APB-5B at week 4,and ACC and CPT-1 at week 8.The results indicated enhanced antioxidant activities at week 8,as evidenced by reduced hepatic malondialdehyde(MDA)level and improved antioxidant enzymes activities in PM and Succinate groups(P＜0.05).Supplementing with Prevotella or succinate can alter the cecal microbiota.Specifically,the abundance of Prevotella in the Succinate group was significantly higher than that in the other 4 groups at the family and genus levels(P＜0.05).Conclusions Oral intake of Prevotella and dietary supplementation of succinate can ameliorate lipid metabolism of laying hens.The beneficial effect of Prevotella is consistent across different species.The finding highlights that suc-cinate,the primary metabolite of Prevotella,represents a more feasible feed additive for alleviating fatty liver in laying hens.
基金项目
国家自然科学基金(32330101)
国家自然科学基金(32102575)
国家重点研发计划(2021YFD1300405)
Modern Agroindustry Technology Research System(CARS-40-K9)
Key Technology Research and Development Program of Shandong Province(2019JZZY020602)
山东省自然科学基金(ZR2020QC180)
中国博士后科学基金(2019M660163)
国家科技期刊平台
NSTL
万方数据