High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

Haorong He ¹Yuanhang Wei ¹Yuqi Chen ¹Xiyu Zhao ¹Xiaoxu Shen ¹Qing Zhu ¹Huadong Yin¹

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作者信息

1. Key Laboratory of Livestock and Poultry Multi-Omics,Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan 611130,China;Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu,Sichuan 611130,China
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Abstract

Background The reproductive performance of chickens mainly depends on the development of follicles.Abnor-mal follicle development can lead to decreased reproductive performance and even ovarian disease among chick-ens.Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer.In recent years,the involvement of circRNAs in follicle development and atresia regulation has been confirmed.Results In the present study,we used healthy and atretic chicken follicles for circRNA RNC-seq.The results showed differential expression of circRALGPS2.It was then confirmed that circRALGPS2 can translate into a protein,named cir-cRALGPS2-212aa,which has IRES activity.Next,we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1.Conclusions Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

Key words

Apoptosis/Autophagy/Chicken/CircRALGPS2/Follicle atresia/PARP1/RNC-seq

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基金项目

National Key Research and Development Program of China(2021YFD1300600)

China Agriculture Research System of MOF and MARA(CARS-40)

Sichuan Science and Technology Program(2021YFYZ0007)

Sichuan Science and Technology Program(2021YFYZ0031)

Sichuan Science and Technology Program(2022YFYZ0005)

National Natural Science Foundation of China Grants(31972543)

出版年

2024

畜牧与生物技术杂志(英文版)

中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD

影响因子：0.765

ISSN：1674-9782

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