首页|Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

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Background In poultry,the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry.However,the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains unclear.Results Critical biochemical events involved in ovulation in domestic chickens(Gallus gallus)were evaluated by transcriptomics,proteomics,and in vitro assays.Comparative transcriptome analyses of the largest preovulatory follicle(F1)and postovulatory follicle(POF1)in continuous laying(CL)and intermittent laying(IL)chickens indicated the greatest difference between CL_F1 and IL_F1,with 950 differentially expressed genes(DEGs),and the smallest difference between CL_POF1 and IL_POF1,with 14 DEGs.Additionally,data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1.Perivitelline membrane synthesis,steroid biosynthesis,lysosomes,and oxidative phosphorylation were identifiied as pivotal pathways contributing to ovulation regulation.In particular,the regulation of zona pellucida sperm-binding protein 3,plasminogen activator,cathepsin A,and lactate dehydrogenase A(LDHA)was shown to be essential for ovulation.Furthermore,the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in vitro.Conclusions This study reveals several important biochemical events involved in the process of ovulation,as well as crucial role of LDHA.These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

ChickenData-independent acquisition proteomicsLDHAOvulationRegulatory mechanismTranscriptome

Ruixue Nie、Wenhui Zhang、Haoyu Tian、Junying Li、Yao Ling、Bo Zhang、Hao Zhang、Changxin Wu

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State Key Laboratory of Animal Biotech Breeding,Beijing Key Laboratory for Animal Genetic Improvement,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China

National Key Research and Development Program of ChinaKey Research and Development Program of ShandongChina Agriculture Research System

2022YFD16009022022LZGC013CARS-40

2024

10.1186/s40104-024-01019-2

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(4)