Abstract
Background Carcass traits are crucial indicators of meat production efficiency.However,the molecular regulatory mechanisms associated with these traits remain unclear.Results In this study,we conducted comprehensive transcriptomic and genomic analyses on 399Tiannong par-tridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regu-latory mechanisms.Based on association analyses with the elastic net(EN)model,we identified 12 candidate genes(AMY1A,AP3B2,CEBPG,EEF2,EIF4EBP1,FGFR1,FOXD3,GOLM1,LOC107052698,PABPC1,SERPINB6 and TBC1D16)for 4 carcass-related traits,namely live weight,dressed weight,eviscerated weight,and breast muscle weight.SERPINB6 was identified as the only overlapping gene by 3 analyses,EN model analysis,weighted gene co-expression network analysis and differential expression analysis.Cell-level experiments confirmed that SERPINB6 promotes the prolifera-tion of chicken DF1 cellsand primary myoblasts.Further expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 expression.Furthermore,a dual-lucif-erase reporter assay proved that gga-miR-1615 targets the 3'UTR ofSERPINB6.Conclusions Collectively,our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by pro-moting fibroblast and myoblast proliferation.Additionally,the downstream variant rs317934171 regulates SERPINB6 expression.These results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.
基金项目
Key Projects of National Natural Science Foundation of China(32230101)
Project of Qingyuan Science and Technology(2020A01)
Project of Qingyuan Science and Technology(2021SJXM011)
Agriculture Research System(CARS-41)
Core Breed Source Research Project(JBGS2021107)
万方数据