首页|Large-scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits
Large-scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits
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Background Carcass traits are crucial indicators of meat production efficiency.However,the molecular regulatory mechanisms associated with these traits remain unclear.Results In this study,we conducted comprehensive transcriptomic and genomic analyses on 399Tiannong par-tridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regu-latory mechanisms.Based on association analyses with the elastic net(EN)model,we identified 12 candidate genes(AMY1A,AP3B2,CEBPG,EEF2,EIF4EBP1,FGFR1,FOXD3,GOLM1,LOC107052698,PABPC1,SERPINB6 and TBC1D16)for 4 carcass-related traits,namely live weight,dressed weight,eviscerated weight,and breast muscle weight.SERPINB6 was identified as the only overlapping gene by 3 analyses,EN model analysis,weighted gene co-expression network analysis and differential expression analysis.Cell-level experiments confirmed that SERPINB6 promotes the prolifera-tion of chicken DF1 cellsand primary myoblasts.Further expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 expression.Furthermore,a dual-lucif-erase reporter assay proved that gga-miR-1615 targets the 3'UTR ofSERPINB6.Conclusions Collectively,our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by pro-moting fibroblast and myoblast proliferation.Additionally,the downstream variant rs317934171 regulates SERPINB6 expression.These results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Life Science and Engineering,Foshan University,Foshan,China
State Key Laboratory of Animal Biotech Breeding,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing,China
Guangdong Tinoo's Foods Group Co.,Ltd.,Qingyuan,China
Key Projects of National Natural Science Foundation of ChinaProject of Qingyuan Science and TechnologyProject of Qingyuan Science and TechnologyAgriculture Research SystemCore Breed Source Research Project
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