首页|Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes
Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes
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Background Pork quality is affected by the type of muscle fibers,which is closely related to meat color,tender-ness and juiciness.Exosomes are tiny vesicles with a diameter of approximately 30-150 nm that are secreted by cells and taken up by recipient cells to mediate communication.Exosome-mediated muscle-fat tissue crosstalk is a newly discovered mechanism that may have an important effect on intramuscular fat deposition and with that on meat quality.Various of adipose tissue-derived exosomes have been discovered and identified,but the identification and function of muscle exosomes,especially porcine fast/slow myotube exosomes,remain unclear.Here,we first isolated and identified exosomes secreted from porcine extensor digitorum longus(EDL)and soleus(SOL),which represent fast and slow muscle,respectively,and further explored their effects on lipid accumulation in longissimus dorsi adipocytes.Results Porcine SOL-derived exosomes(SOL-EXO)and EDL-derived exosomes(EDL-EXO)were first identified and their average particle sizes were approximately 84 nm with double-membrane disc-shapes as observed via transmission electron microscopy and scanning electron microscopy.Moreover,the intramuscular fat content of the SOL was greaterthan that of the EDL at 180 days of age,because SOL intramuscular adipocytes had a stronger lipid-accumulating capacity than those of the EDL.Raman spectral analysis revealed that SOL-EXO protein content was much greater than that of EDL-EXO.Proteomic sequencing identified 72 proteins that were significantly differen-tially expressed between SOL-EXO and EDL-EXO,31 of which were downregulated and 41 of which were upregulated in SOL-EXO.Conclusions Our findings suggest that muscle-fat tissue interactions occur partly via SOL-EXO promoting adipo-genic activity of intramuscular adipocytes.
Laboratory of Animal Fat Deposition & Muscle Development,College of Ani-mal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi,China
Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Chang-sha 410125,Hunan,China
National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaKey Research and Development Program of Shaanxi ProvinceChina Agriculture Research System of MOF and MARA
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