首页|Sperm function,mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

Sperm function,mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

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Background Despite their low abundance in sperm,mitochondria have diverse functions in this cell type,includ-ing energy production,signalling and calcium regulation.In humans,sperm mitochondrial DNA content(mtDNAc)has been reported to be negatively linked to sperm function and fertility.Yet,the association between mtDNAc and sperm function in livestock remains unexplored.For this reason,this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs.A qPCR method for mtDNAc quantification was optimised for pig sperm,and the association of this parameter with sperm motility,kinematics,mitochondrial activity,and fertility was subsequently interrogated.Results First,the qPCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm.By using this technique,mtDNAc was observed to be associated to sperm motility,mitochondrial activity and in vivo,but not in vitro,fertility outcomes.Specifically,sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species.Interestingly,samples with lower mtD-NAc showed higher conception and farrowing rates,but similar in vitro fertilisation rates and embryo development,when compared to those with greater mtDNAc.Conclusions These findings enrich our comprehension of the association of mtDNAc with sperm biology,and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

FertilityMitochondriaMotilitymtDNA copy numberqPCRSperm

Marc Llavanera、Yentel Mateo-Otero、Estel Vi?olas-Vergés、Sergi Bonet、Marc Yeste

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Unit of Cell Biology,Department of Biology,Faculty of Sciences,University of Girona,Girona ES-17003,Spain

Biotechnology of Animal and Human Reproduction(TechnoSperm),Institute of Food and Agricultural Technology,University of Girona,Girona ES-17003,Spain

Catalan Institution for Research and Advanced Studies(ICREA),Barcelona ES-08010,Spain

Ministry of Science and Innovation,SpainMinistry of Science and Innovation,SpainMinistry of Science and Innovation,SpainRegional Government of Catalonia,SpainRegional Government of Catalonia,SpainRegional Government of Catalonia,SpainCatalan Institution for Research and Advanced Studies(ICREA)

AGL2017-88329-RFPU18/00666PID2020-113320RB-I002017-SGR-12292020-FI-B-004122020-SGR-0900

2024

10.1186/s40104-023-00988-0

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(4)