Abstract
Transfer RNA-derived small RNAs(tsRNAs)have been shown to be involved in early embryo development and repres-sion of endogenous retroelements in embryos and stem cells.However,it is unknown whether tsRNAs also regulate embryo hatching.In this study,we mined the sequencing data of a previous experiment in which we demonstrated that the microRNA(miRNA)cargo of preimplantation embryonic extracellular vesicles(EVs)influences embryo development.We thus profiled the tsRNA cargo of EVs secreted by blastocysts and non-blastocysts.The majority of tsRNAs was identified as tRNA halves originating from the 5'ends of tRNAs.Among the 148 differentially expressed tsRNAs,the 19 nt tRNA fragment(tRF)tDR-14∶32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from non-blastocysts.RT-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group(P＜0.05).Inhibition of tDR-14∶32-Glu-CTC-1 by supplement-ing antagomirs to the conditioned medium improved embryo hatching(P＜0.05).Transcriptomic analysis of embryos treated with tDR-14∶32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and implantation.In summary,tDR-14∶32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions,and inhibition of tDR-14∶32-Glu-CTC-1 promotes embryo hatching,while influencing embryo implantation-related genes and pathways.These results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.
基金项目
Ghent University(2018000504[GOA030-18 BOF])
China Scholarship Council(202006910034)
Ghent University Fonds Wetenschappelijk Onderzoek(BOF.STG.2022.02.0034.01)
Ghent University Fonds Wetenschappelijk Onderzoek(1228821N)
Ghent University Fonds Wetenschappelijk Onderzoek(12A2H24N)
万方数据