首页|Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

扫码查看
原文链接
  • 万方数据
  • 维普
Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food pollution.The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear.This study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.Methods A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were ran-domly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur group.Each treatment consisted of 3 replicates(180 fish)for 60 d.Results Firstly,we cultured,purified,and identified myoblasts using the tissue block culture method.Through pre-liminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect.Secondly,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(MyoG and MYHC)in vivo and in vitro and improve the growth performance of grass carp.Then,Cur could also promote the expression of OTA-inhibited protein syn-thesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling pathway.Finally,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the FoxO3a signaling pathway.Conclusions In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro.This study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

CurcuminGrass carpMyotoxicOchratoxin AProtein deposition

Piao Zhao、Lin Feng、Weidan Jiang、Pei Wu、Yang Liu、Hongmei Ren、Xiaowan Jin、Lu Zhang、Haifeng Mi、Xiaoqiu Zhou

展开 >

Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Fish Nutrition and Safety Production University Key Labora-tory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Key Laboratory of Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu 611130,Sichuan,China

Tongwei Co.,Ltd.,Healthy Aquaculture Key Laboratory of Sichuan Province,Chengdu 610041,Sichuan,China

展开 >

earmarked fund for CARSNational Natural Science Foundation of China for Outstanding Youth Science FoundationYoung Top-Notch Talent Support Program111 project

CARS-4531922086D17015

2024

10.1186/s40104-024-01023-6

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(4)