Abstract
Understanding biological mechanisms is fundamental for improving animal production and health to meet the grow-ing demand for high-quality protein.As an emerging biotechnology,single-cell transcriptomics has been gradually applied in diverse aspects of animal research,offering an effective method to study the gene expression of high-throughput single cells of different tissues/organs in animals.In an unprecedented manner,researchers have identi-fied cell types/subtypes and their marker genes,inferred cellular fate trajectories,and revealed cell-cell interactions in animals using single-cell transcriptomics.In this paper,we introduce the development of single-cell technology and review the processes,advancements,and applications of single-cell transcriptomics in animal research.We sum-marize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and health,reproductive performance,genetics,and disease models in different livestock species.Moreover,the prac-tical experience accumulated based on a large number of cases is highlighted to provide a reference for determin-ing key factors(e.g.,sample size,cell clustering,and cell type annotation)in single-cell transcriptomics analysis.We also discuss the limitations and outlook of single-cell transcriptomics in the current stage.This paper describes the comprehensive progress of single-cell transcriptomics in animal research,offering novel insights and sustainable advancements in agricultural productivity and animal health.
国家科技期刊平台
NSTL
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