首页|From follicle to blastocyst:microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality

From follicle to blastocyst:microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality

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From follicle to blastocyst:microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality
Background Within the follicular fluid,extracellular vesicles(EVs)guide oocyte growth through their cargo micro-RNAs(miRNAs).Here,we investigated the role of EVs and their cargo miRNAs by linking the miRNAs found in EVs,derived from the fluid of an individual follicle,to the ability of its oocyte to become a blastocyst(competent)or not(non-competent).Methods Bovine antral follicles were dissected,categorized as small(2-4 mm)or large(5-8 mm)and the corre-sponding oocytes were subjected to individual maturation,fertilization and embryo culture to the blastocyst stage.Follicular fluid was pooled in 4 groups(4 replicates)based on follicle size and competence of the corresponding oocyte to produce a blastocyst.Follicular fluid-derived EVs were isolated,characterized,and subjected to miRNA-sequencing(Illumina Miseq)to assess differential expression(DE)in the 4 groups.Functional validation of the effect of miR-34c on embryo development was performed by supplementation of mimics and inhibitors during in vitro maturation(IVM).Results We identified 16 DE miRNAs linked to oocyte competence when follicular size was not considered.Within the large and small follicles,46 DE miRNAs were driving blastocyst formation in each group.Comparison of EVs from competent small and large follicles revealed 90 DE miRNAs.Cell regulation,cell differentiation,cell cycle,and metabolic process regulation were the most enriched pathways targeted by the DE miRNAs from competent oocytes.We identified bta-miR-34c as the most abundant in follicular fluid containing competent oocytes.Supple-mentation of miR-34c mimic and inhibitor during IVM did not affect embryo development.However,blastocyst qual-ity,as evidenced by higher cell numbers,was significantly improved following oocyte IVM in the presence of miR-34c mimics,while miR-34c inhibitors resulted in the opposite effect.Conclusion This study demonstrates the regulatory effect of miRNAs from follicular fluid-derived EVs on oocyte competence acquisition,providing a further basis for understanding the significance of miRNAs in oocyte maturation and embryonic development.Up-regulation of miR-34c in EVs from follicular fluid containing competent oocytes and the positive impact of miR-34c mimics added during IVM on the resulting blastocysts indicate its pivotal role in oocyte competence.

Developmental competenceExtracellular vesiclesMaturationMicroRNAmiR-34cOocyte

Camilla Benedetti、Krishna Chaitanya Pavani、Yannick Gansemans、Nima Azari-Dolatabad、Osvaldo Bogado Pascottini、Luc Peelman、Rani Six、Yuan Fan、Xuefeng Guan、Koen Deserranno、Andrea Fernández-Montoro、Joachim Hamacher、Filip Van Nieuwerburgh、Trudee Fair、An Hendrix、Katrien Smits、Ann Van Soom

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Department of Internal Medicine,Reproduction and Population Medicine,Faculty of Veterinary Medicine,Ghent University,9820 Merelbeke,Bel-gium

Department for Reproductive Medicine,Ghent University Hospital,9000 Ghent,Belgium

Laboratory of Pharmaceutical Biotechnology,Faculty of Pharmaceutical Sciences,Ghent University,B-9000 Ghent,Belgium

Department of Animal Science,University of Tennessee,Knoxville,TN,USA

Department of Nutrition,Genetics and Ethology,Faculty of Veterinary Medicine,Ghent University,B-9000 Ghent,Belgium

Institute of Crop Science and Resource Conservation,Plant Pathology,Rheinische Friedrich-Wilhelms-University of Bonn,53115 Bonn,Germany

School of Agriculture and Food Science,University College Dublin,Belfield,Dublin,Ireland

Laboratory of Experimental Cancer Research,Department of Human Structure and Repair,Ghent University,B-9000 Ghent,Belgium

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Developmental competence Extracellular vesicles Maturation MicroRNA miR-34c Oocyte

2024

10.1186/s40104-024-01059-8

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(6)