首页|Visfatin(NAMPT)affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle
Visfatin(NAMPT)affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle
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国家科技期刊平台
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Visfatin(NAMPT)affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle
Background The pituitary belongs to the most important endocrine glands involved in regulating reproductive functions.The proper functioning of this gland ensures the undisturbed course of the oestrous cycle and affects the female's reproductive potential.It is believed that visfatin,a hormone belonging to the adipokine family,may regulate reproductive functions in response to the female's metabolic state.Herein we verified the hypothesis that suggests a modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle.Results RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 202 genes(95 up-regulated and 107 down-regulated in the presence of visfatin,when compared to the non-treated controls),assigned to 318 gene ontology terms.We revealed changes in the frequency of alternative splicing events(235 cases),as well as long noncoding RNA expression(79 cases)in the presence of the adipokine.The identified genes were associated,among others,with reproductive system development,epithelial cell proliferation,positive regulation of cell development,gland morphogenesis and cell chemotaxis.Conclusions The obtained results indicate a modulatory influence of visfatin on the regulation of the porcine tran-scriptome and,in consequence,pituitary physiology during the mid-luteal phase of the oestrous cycle.
Alternative splicingAnterior pituitaryDifferentially expressed genesIncRNAOestrous cyclePigRNAseqTranscriptomeVisfatin
国家科技期刊平台
NSTL
万方数据
