首页|Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation
Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation
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Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation
Background Polystyrene nanoplastics(PS-NPs)are becoming increasingly prevalent in the environment with great advancements in plastic products,and their potential health hazard to animals has received much attention.Several studies have reported the toxicity of PS-NPs to various tissues and cells;however,there is a paucity of information about whether PS-NPs exposure can have toxic effects on mammalian oocytes,especially livestock.Herein,porcine oocytes were used as the model to investigate the potential effects of PS-NPs on mammalian oocytes.Results The findings showed that different concentrations of PS-NPs(0,25,50 and 100 μg/mL)entering into porcine oocytes could induce mitochondrial stress,including a significant decrease in mitochondrial membrane potential(MMP),and the destruction of the balance of mitochondrial dynamic and micromorphology.Furthermore,there was a marked increase in reactive oxygen species(ROS),which led to oocyte lipid peroxidation(LPO).PS-NPs expo-sure induced abnormal intracellular iron overload,and subsequently increased the expression of transferrin receptor(TfRC),solute carrier family 7 member 11(SLC7a11),and acyl-CoA synthetase long-chain family member 4(ACSL4),which resulted in ferroptosis in oocytes.PS-NPs also induced oocyte maturation failure,cytoskeletal dysfunction and DNA damage.Cotreatment with 5 μmol/L ferrostatin-1(Fer-1,an inhibitor of ferroptosis)alleviated the cellular toxicity associated with PS-NPs exposure during porcine oocyte maturation.Conclusions In conclusion,PS-NPs caused ferroptosis in porcine oocytes by increasing oxidative stress and altering lipid metabolism,leading to the failure of oocyte maturation.
Yijing He、Tian hang Yu、Heran Li、Qinfeng Sun、Miaoyu Chen、Yiyi Lin、Jianjun Dai、Weihan Wang、Qiao Li、Shiqiang Ju
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MOE Joint International Research Laboratory of Animal Health and Food Safety,College of Veterinary Medicine,Nanjing Agricultural University,Nan-jing 210095,China
Key Laboratory of Livestock and Poultry Resources(Pig)Evaluation and Utilization,Ministry of Agriculture and Rural Affairs,Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricul-tural Sciences,Shanghai 201106,China
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