首页|Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway

Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway

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  • 国家科技期刊平台
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Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway
Background Follicular cysts contribute significantly to reproductive loss in high-yield dairy cows.This results from the death of follicular granulosa cells(GCs)caused by oxidative stress.Quercetin is known to have significant antioxidant and anti-apoptotic effects.However,the effect of quercetin on follicular cysts has yet been elucidated.Therefore,this study aimed to explore the anti-oxidant and anti-apoptosis effects and potential molecular mecha-nisms of quercetin in H2O2-induced primary cow GCs and 3-nitropropionic acid(3-NPA)-induced mouse model of oxi-dative stress and thus treat ovarian cysts in dairy cows.Results In this study,compared with estrus cows,cows with follicular cysts showed heightened levels of oxida-tive stress and increased follicular cell apoptosis,while autophagy levels were reduced.A model of oxidative stress was induced in vitro by H2O2 and showed significant increases in apoptosis together with reduced autophagy.These effects were significantly ameliorated by quercetin.Effects similar to those of quercetin were observed after treat-ment of cells with the reactive oxygen species(ROS)inhibitor N-acetylcysteine(NAC).Further investigations using chloroquine(autophagy inhibitor),rapamycin(autophagy activator),selisistat(SIRT1 inhibitor),and compound C(AMPK inhibitor)showed that chloroquine counteracted the effects of quercetin on oxidative stress-induced apop-tosis,while rapamycin had the same effect as quercetin.In addition,the SIRT1/AMPK pathway inhibitors antagonized quercetin-mediated mitigation of the effects of oxidative stress on increased apoptosis and reduced autophagy.Consistent with the results in vitro,in mouse ovarian oxidative stress model induced by 3-NPA,quercetin activated autophagy through the SIRT1/AMPK signaling pathway,while alleviating oxidative stress damage and inhibiting apoptosis in mouse ovaries.Conclusions These findings indicate that quercetin can inhibit apoptosis in GCs and restore ovarian function by acti-vating autophagy through the SIRT1/ROS/AMPK signaling pathway,suggesting a new direction for the treatment of ovarian follicular cysts in high-yield dairy cows.

ApoptosisAutophagyFollicular cystOxidative stressQuercetin

Hongwei Duan、Fang Wang、Ke Wang、Shuai Yang、Rong Zhang、Chen Xue、Lihong Zhang、Xiaofei Ma、Xianghong Du、Jian Kang、Yong Zhang、Xingxu Zhao、Junjie Hu、Longfei Xiao

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College of Veterinary Medicine,Gansu Agricultural University,Lan-zhou 730070,Gansu,China

Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation,Lanzhou 730070,Gansu,China

Gansu Institute of Animal Husbandry and Veterinary,Pingliang 744000,Gansu,China

School of Animal Science and Technology,Guangdong Polytechnic of Science and Trade,Guangzhou 510640,Guangdong,China

Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China

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Apoptosis Autophagy Follicular cyst Oxidative stress Quercetin

2024

10.1186/s40104-024-01078-5

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(6)