首页|Epigallocatechin-3-gallate protects bovine ruminal epithelial cells against lipopolysaccharide-induced inflammatory damage by activating autophagy

Epigallocatechin-3-gallate protects bovine ruminal epithelial cells against lipopolysaccharide-induced inflammatory damage by activating autophagy

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Epigallocatechin-3-gallate protects bovine ruminal epithelial cells against lipopolysaccharide-induced inflammatory damage by activating autophagy
Background Subacute ruminal acidosis(SARA)causes an increase in endotoxin,which can induce immune and inflammatory responses in the ruminal epithelium of dairy cows.In non-ruminants,epigallocatechin-3-gallate(EGCG),a major bioactive ingredient of green tea,is well-known to alleviate inflammation.Whether EGCG confers protection against SARA-induced inflammation and the underlying mechanisms are unknown.Results In vivo,eight ruminally cannulated Holstein cows in mid-lactation were randomly assigned to either a low-concentrate(40％)diet(CON)or a high-concentrate(60％)diet(HC)for 3 weeks to induce SARA(n=4).Cows with SARA had greater serum concentrations of tumor necrosis factor(TNF)-α and interleukin-6,and epithelium had histological signs of damage.In vitro,immortalized bovine ruminal epithelial cells(BREC)were treated with lipopoly-saccharide(LPS)to imitate the inflammatory damage caused by SARA.Our data revealed that BREC treated with 10 μg/mL LPS for 6 h successfully induce a robust inflammatory response as indicated by increased phosphoryla-tion of IKBα and nuclear factor kappa-B(NF-κB)p65.Pre-treatment of BREC with 50 μmol/L EGCG for 6 h before LPS challenge promoted the degradation of NLR family pyrin domain containing 3(NLRP3)inflammasome through acti-vation of autophagy,which further repressed activation of NF-κB pathway targeting Toll-like receptor 4(TLR4).Analy-ses also revealed that the ECGG upregulated tight junction(TJ)protein expression upon incubation with LPS.Conclusions Subacute ruminal acidosis causes ruminal epithelium injury and systemic inflammation in dairy cows.However,the anti-inflammatory effects of EGCG help preserve the integrity of the epithelial barrier through activat-ing autophagy when BREC are exposed to LPS.Thus,EGCG could potentially serve as an effective therapeutic agent for SARA-associated inflammation.

Bovine ruminal epithelial cellEpigallocatechin-3-gallateInflammationSubacute ruminal acidosis

Wanli Zhao、Taiyu Shen、Bichen Zhao、Moli Li、Zhaoju Deng、Yihui Huo、Ben Aernouts、Juan J.Loor、Androniki Psifidi、Chuang Xu

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National Key Laboratory of Veterinary Public Health and Safety,College of Veterinary Medicine,China Agricultural University,2 Yuanmingyuan West Road,Beijing 100193,China

Department of Biosystems,Division of Animal and Human Health Engineering,KU Leuven University,Kleinhoefstraat 4,Geel 2440,Belgium

Department of Animal Sciences,Division of Nutritional Sciences,University of Illinois,Urbana-Champaign,Urbana,IL 61801,USA

Department of Clinical Science and Services,Queen Mother Hospital for Ani-mals,The Royal Veterinary College,North Mymms,Hawkshead Lane,Hatfield,Hertfordshire AL9 7TA,UK

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Bovine ruminal epithelial cell Epigallocatechin-3-gallate Inflammation Subacute ruminal acidosis

2024

10.1186/s40104-024-01066-9

畜牧与生物技术杂志(英文版)
中国科学技术协会

畜牧与生物技术杂志(英文版)

CSTPCD
影响因子：0.765
ISSN：1674-9782
年,卷(期)：2024.15(6)