首页|Effects of grain intervention on hypothalamic function and the metabolome of blood and milk in dairy cows
Effects of grain intervention on hypothalamic function and the metabolome of blood and milk in dairy cows
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国家科技期刊平台
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Effects of grain intervention on hypothalamic function and the metabolome of blood and milk in dairy cows
Background The hypothalamus plays a crucial role in the health and productivity of dairy cows,yet studies on its functionality and its impact on peripheral circulation in these animals are relatively scarce,particularly regard-ing dietary interventions.Therefore,our study undertook a comprehensive analysis,incorporating both metabolomics and transcriptomics,to explore the effects of a grain-based diet on the functionality of the hypothalamus,as well as on blood and milk in dairy cows.Results The hypothalamic metabolome analysis revealed a significant reduction in prostaglandin E2(PGE2)level as a prominent response to the grain-based diet introduction.Furthermore,the hypothalamic transcriptome profiling showed a notable upregulation in amino acid metabolism due to the grain-based diet.Conversely,the grain-based diet led to the downregulation of genes involved in the metabolic pathway from lecithin to PGE2,including phospho-lipase A2(PLA2G4E,PLA2G2A,and PLA2G12B),cyclooxygenase-2(COX2),and prostaglandin E synthase(PTGES).Addi-tionally,the plasma metabolome analysis indicated a substantial decrease in the level of PGE2,along with a decline in adrenal steroid hormones(tetrahydrocortisol and pregnenolone)following the grain-based diet introduction.Analysis of the milk metabolome showed that the grain-based diet significantly increased uric acid level while notably decreasing PGE2 level.Importantly,PGE2 was identified as a critical metabolic marker in the hypothalamus,blood,and milk in response to grain intervention.Correlation analysis demonstrated a significant correlation among meta-bolic alterations in the hypothalamus,blood,and milk following the grain-based diet.Conclusions Our findings suggest a potential link between hypothalamic changes and alterations in peripheral circulation resulting from the introduction of a grain-based diet.
Limei Lin、Kaizhen Guo、Huiting Ma、Jiyou Zhang、Zheng Lai、Weiyun Zhu、Shengyong Mao
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Laboratory of Gastrointestinal Microbiology,Jiangsu Key Laboratory of Gas-trointestinal Nutrition and Animal Health,National Center for International Research On Animal Gut Nutrition,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
Ruminant Nutrition and Feed Engineering Technology Research Center,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
国家科技期刊平台
NSTL
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