首页|Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profile
Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profile
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国家科技期刊平台
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Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profile
Background Magnolol(MAG)exhibits hepatoprotective activity,however,whether and how MAG regulates the gut microbiota to alleviate fatty liver hemorrhagic syndrome(FLHS)remains unclear.Therefore,we investigated the mech-anism of MAG in FLHS laying hens with an emphasis on alterations in the gut-liver axis.We randomly divided 54056-week-old Hy-line white laying hens with FLSH into 4 groups.The birds were fed a high-fat low-protein(HFLP)diet(CON)or HELP diets supplemented with 200,400,and 600 mg/kg of MAG(M1,M2,and M3,respectively)for 9 weeks.Results Magnolol supplementation increased the laying rate and ameliorated hepatic damage and dysfunction by regulating lipid metabolism,improving intestinal barrier function,and shaping the gut microbiota and tryptophan metabolic profiles.Dietary MAG supplementation downregulated the expression of lipid synthesis genes and upregu-lated the expression of lipid transport genes at varying degrees.The intestinal barrier function was improved by 200 and 400 mg/kg of MAG supplementation,as evidenced by the increased villus height and mRNA expression of tight junction related genes.Microbiological profile information revealed that MAG changed the gut microbiota,espe-cially by elevating the abundances of Lactobacillus,Faecalibacterium,and Butyricicoccus.Moreover,non-targeted metabolomic analysis showed that MAG significantly promoted tryptophan metabolites,which was positively correlated with the MAG-enriched gut microbiota.The increased tryptophan metabolites could activate aryl hydro-carbon receptor(AhR)and relieved hepatic inflammation and immune response evidenced by the downregulated the gene expression levels of pro-inflammatory cytokines such as interleukin-1(3(IL-1β),tumor necrosis factor-a(TNF-a),and interleukin-6(IL-6)in the liver.The fecal microbiota transplantation(FMT)experiments further confirmed that the hepatoprotective effect is likely mediated by MAG-altered gut microbiota and their metabolites.Conclusions Magnolol can be an outstanding supplement for the prevention and mitigation of FLHS in laying hens by positively regulating lipid synthesis and transport metabolism,improving the intestinal barrier function,and reliev-ing hepatic inflammation by reshaping the gut microbiota and metabolite profiles through gut microbiota-indole metabolite-hepatic AhR crosstalk.These findings elucidate the mechanisms by which MAG alleviates FLHS and pro-vide a promising method for preventing liver diseases by modulating gut microbiota and their metabolites.
国家科技期刊平台
NSTL
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