Antibody dynamics of Brucella vaccine S2 in the immunized sheep
In order to understand the serum antibody dynamics in sheep immunized with brucellosis vaccine S2 by oral and injection methods,the antibody levels were assayed using the tiger red plate agglutination test (RBT),tube agglutination test (SAT) and competitive enzyme linked immunosorbent assay (cELISA) methods.The results showed that the serum antibody positive rates reached the highest level using normal dosage of oral brucellosis vaccine S2 at 20d after immunization,with RBT 80％,SAT 70％ and cELISA 60％,respectively;after 100d,serum antibody positive rates were RBT 20％,SAT 10％ and cELISA 10％;after 250d,the serum antibodies were all negative by three methods.At 20d after immunization,RBT,SAT and cELISA antibodies reached as high as 60％ using the double dosage of oral vaccine S2,and all became negative after 100d.The antibody positive rates reached the highest level (80％) by RBT and SAT tests using normal dosage for injection at 7d after vaccination,and cELISA tests reached 60％ and then 80％ after 30d;however,after 370d,RBT and SAT antibody positive rates were decreased to 60％,while the cELISA antibody positive rate was still 80％.Using the double dosage of injection the RBT and cELISA antibody positive rates still maintained 100％,while SAT antibody positive rate was 80％ at 370d after immunization.From antibody production and dynamics in the immunized sheep,the efficacy of brucellosis vaccine S2 administrated by injective route was obviously better than that by oral route,and whether oral immunization or injection,no significant side effects were found.
国家科技期刊平台
NSTL
万方数据
维普
