首页|糯谷猪PRKAA1基因表达与肉用性状关联研究

糯谷猪PRKAA1基因表达与肉用性状关联研究

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旨在掌握贵州糯谷猪胴体和肉质性状表型差异,分析单磷酸腺苷活化蛋白激酶α1亚基(PRKAA1)基因mRNA表达量和肉用性状关联性.以糯谷猪为研究对象,分别对10月龄的9头去势公猪和10头母猪进行屠宰测定,采用实时荧光定量PCR检测糯谷猪背最长肌组织PRKAA1基因mRNA表达量,并与测定性状进行相关性分析.结果:去势公猪和母猪宰前活重分别为(94.78±8.75)kg和(93.10±9.23)kg,两者差异不显著(P>0.05),但母猪皮率和瘦肉率指标分别高于去势公猪21.91%和8.74%,且差异显著(P<0.05),肥肉率指标极显著低于去势公猪(P<0.01);关联分析发现,糯谷猪母猪背最长肌组织PRKAA1基因mRNA表达水平与肩部最厚处背膘厚呈极显著负相关性(P<0.01).本研究分析了性别对糯谷猪肉用性状的影响,发现PRKAA1可作为调控糯谷猪胴体性状的候选基因.
Correlation between PRKAA1 gene expression level and meat traits in Nuogu pigs
This study was to understand the phenotypic differences in carcass and meat quality traits of Guizhou Nuogu pigs,and to analyze the correlation between PRKAA1 gene mRNA expression and meat quality traits.Nuogu pigs were selected as the research objects.The car-cass and meat quality indexes of 9 castrated boars and 10 sows of Nuogu pigs were determined,and the mRNA expression level of the longis-simus dorsi muscle tissue of the pigs was detected by real-time quantitative PCR(RT-qPCR),and the correlation between their PRKAA1 gene mRNA expression level and meat quality traits was analyzed.The results showed that there were no significant differences in pre-slaugh-ter live weight between the castrated boars(94.78±8.75)kg and sows(93.10±9.23)kg.The indexes of skin percentage and lean meat percentage of the sows were significantly higher than those of the castrated boars by 21.91%and 8.74%(P<0.05),respectively;but their fat meat percentage was extreme significantly lower than that of the castrated boars(P<0.01).The correlation analysis showed that there was a significant negative correlation between the PRKAA1 gene mRNA expression level of the longissimus dorsalis muscle at the back and back-fat thickness at the thickest part of the shoulder of the sows(P<0.01).In summary,this study analyzed the effect of gender on the meat per-formance of Nuogu pigs and confirmed that PRKAA1 could be used as a candidate gene for regulating the carcass traits of Nuogu pigs.

NuogupigPRKAA1 genecarcassmeat

张雄、史开志、杨齐心、周思旋、吴玙彤、李冬光、赵春萍

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贵州省农业科学院畜牧兽医研究所,贵州贵阳 550005

贵州省畜禽遗传资源管理站,贵州贵阳 550004

糯谷猪 PRKAA1基因 胴体 肉质

2025

畜牧与兽医
南京农业大学

畜牧与兽医

北大核心
影响因子:0.404
ISSN:0529-5130
年,卷(期):2025.57(1)