Correlation between PRKAA1 gene expression level and meat traits in Nuogu pigs
This study was to understand the phenotypic differences in carcass and meat quality traits of Guizhou Nuogu pigs,and to analyze the correlation between PRKAA1 gene mRNA expression and meat quality traits.Nuogu pigs were selected as the research objects.The car-cass and meat quality indexes of 9 castrated boars and 10 sows of Nuogu pigs were determined,and the mRNA expression level of the longis-simus dorsi muscle tissue of the pigs was detected by real-time quantitative PCR(RT-qPCR),and the correlation between their PRKAA1 gene mRNA expression level and meat quality traits was analyzed.The results showed that there were no significant differences in pre-slaugh-ter live weight between the castrated boars(94.78±8.75)kg and sows(93.10±9.23)kg.The indexes of skin percentage and lean meat percentage of the sows were significantly higher than those of the castrated boars by 21.91%and 8.74%(P<0.05),respectively;but their fat meat percentage was extreme significantly lower than that of the castrated boars(P<0.01).The correlation analysis showed that there was a significant negative correlation between the PRKAA1 gene mRNA expression level of the longissimus dorsalis muscle at the back and back-fat thickness at the thickest part of the shoulder of the sows(P<0.01).In summary,this study analyzed the effect of gender on the meat per-formance of Nuogu pigs and confirmed that PRKAA1 could be used as a candidate gene for regulating the carcass traits of Nuogu pigs.