Effect of the combination of Escherichia coli phage 199 and clindamycin hydrochloride on bactericide in vitro
The aim of this study was to investigate the effect of Escherichia coli bacteriophage Φ199 combined with clindamycin hydrochlo-ride on bactericide I.The biological characteristics of bacteriophage Φ199 with Escherichia coli O157:H7 as the host bacterium were stud-ied,and the antibacterial effect of bacteriophage Φ199 combined with clindamycin hydrochloride was evaluated.The results showed that phage Φ199 was a muscle-tailed bacteriophage with a head diameter of about 50 nm and a tail length of about 100 nm.Bacteriophage Φ199 could survive stably at 40-60 ℃ and maintain lysis activity in the pH range of 3-12,indicating that phage Φ199 had a wide temperature tol-erance and good acid-base tolerance.The optimal MOI of phage Φ199 was 1,and the one-step growth curve showed that the incubation peri-od was 5 min,the lysis period was 5 min,and the lysis amount was 44.The phage was able to inhibit the growth of bacteria within 3 h.In addition,the results of the antibiotic combination showed that the combination of bacteriophage Φ199 and clindamycin hydrochloride had a synergistic effect,which could reduce the effective concentration of antibiotics to 1/2 MIC when combined with the bacteriophage with a titer of 106-107 PFU/mL,and to 1/4 MIC when combined with a titer of 108 PFU/mL.
万方数据
维普
