Protective effect of mulberry leaf polysaccharides on AFB1-induced liver injury
The aim of this study was to explore the protective effect and potential mechanism of mulberry leaf polysaccharides(MLP)on af-latoxin B1(AFB1)induced liver injury in mice.50 Kunming mice were obtained and randomly divided into a blank group,a model group,a positive control group,a low-dose MLP group,and a high-dose MLP group,with 10 mice in each group.The model group was given physio-logical saline(0.1 mL per 10 g of body weight)by gavage,the positive control group was given silymarin(100 mg/kg)by gavage,and the low-dose and high-dose MLP groups were given mulberry leaf polysaccharides(200,and 400 mg/kg,respectively)by gavage.Two hours later,the model group,the positive control group,the low-dose MLP group,and the high-dose MLP group were given AFB1(0.75 mg/kg)by gavage.The blank group was given two doses of physiological saline by gavage(0.1 mL per 10 g of body weight).After continu-ous gastric lavage for 14 days,blood samples were collected from the eye socket of the mice after anesthesia,and the mice were euthanized.Then,the activities of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and alkaline phosphatase(ALP)in the mice were detected.The activities of superoxide dismutase(SOD),glutathione S-transferase(GST),mouse cytochrome CYP4502E1(CYP2E1),and the content of reduced glutathione(GSH)in their serum were also detected in order to evaluate the oxidative stress levels in the rodents.Next,the liver tissue slices were made and the tissue changes in the liver of the animals were observed using the hematoxylin eosin(HE)method.Finally,the QPCR method was used to detect the expression levels of Keap1,Nrf2,and SOD1 mRNA in their liver ho-mogenate.The results showed that,compared with the blank group,the activities of ALT,AST,and ALP in the model group were signifi-cantly increased(P＜0.05).The liver cells were arranged in a disordered manner,the nucleus was severely fragmented,and severe vacuolar degeneration occurred.The activities of GST and SOD were significantly reduced(P＜0.05),the content of GSH was significantly reduced,too(P＜0.05);but the activity of CYP2E1 in the serum was significantly increased(P＜0.05);and the gene expression of Keap1,Nrf2,and SOD1 was significantly reduced(P＜0.05).Compared with the model group,the low-dose and high-dose MLP groups showed lower ac-tivities of ALT,AST,ALP,and CYP2E1 in the serum(P＜0.05),higher activities of GST,SOD,and higher GSH content(P＜0.05);and liver lesions in the mice were improved.It was understood that MLP upregulated the gene expression levels of Nrf2 and SOD1.This study indicated that mulberry leaf polysaccharides provided a pre-protective effect on AFB1 induced liver injury,and its mechanism of action might be that of improving liver antioxidant capacity and regulating the Keap1/Nrf2 signaling pathway.
万方数据
维普
