Establishment and Optimization of in vitro Micropropagation of Rhododendron delavayi Franch
Using seeds of Rhododendron delavayi Franch as the explants,an efficient micropropagation system for this plant species was established and optimized,and ISSR markers were used to assess the genetic stability of the regenerated plantlets.The seeds germinated well in 1/4MS＋1.0 mg/L KT and gave a germination rate of 70% or more.Shoot sections with a terminal bud were the best explants,and WPM（Woody Plant Medium）＋KT 0.5 mg/L＋GA 1.0 mg/L was the optimum medium for bud cluster induction.WPM＋KT 0.5 mg/L＋GA 1.0 mg/L was the optimum medium for the growth of the induced bud clusters.With the addition of 0.5 mg/L KT,1.0 mg/L NAA and 2.0 mg/L IBA,1/2WPM demonstrated the best root induction effect after 30 days,whose rooting rate was as high as 100%.After 90-day acclimatization,80% of the plantlets survived after transplantation.ISSR amplification with 20 primers was made to test the somaclonal variation of the plantlets and no polymorphic bands were detected,thus indicating that this micropropagation system could produce plantlets with high genetic stability.
NETL
NSTL
万方数据
维普
