Anisodus luridus,belonging to the Solanaceae family,is a Tibetan endemic plant species producing pharmaceutical tropane alkaloids. In order to provide a theoretical basis for the scale production of tropane alkaloids with A. luridus hairy roots as the raw material,a study was conducted in which Agrobacterium C58C1 (pRiA4) was used to infect the cotyledon,leaf or stem of A. luridus,and its hairy root induction was recorded. High frequencies of hairy root induction were obtained from stem (60.00%) and leaf (53.33%);while cotyledon produced no hairy roots. Then,the typical hairy root line was used to screen the optimal medium for root culture growth and alkaloid production. Of the seven types of liquid media used in this study (1/2MS,MS,1/2B5,White,WPM,B5 and N6),B5 was the best one for both biomass accumulation and TA production. The fresh weight of hairy root cultures in B5 was 6.9±0.94 g/flask (or 0.44±0.06 g/flask dry weight). Further,HPLC analysis results showed that hairy root cultures in B5 produced hyoscyamine (3.49±0.13 mg/g) at a higher level than that in other media. The highest scopolamine contents were detected in hairy root cultures in B5 and 1/2B5,which were respectively 1.37±0.06 mg/g and 1.43±0.22 mg/g. However,there was no significant difference in scopolamine contents between hairy root cultures in B5 and that in 1/2B5. Finally,it was found that hairy root cultures in B5 produced total TAs at the highest level (2.1±0.29 mg/flask).
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维普
