Genome-wide Analysis of the IQM Gene Family in Rapeseed(Brassica napus L.)
IQM(IQ-Motif Containing)gene family is a Ca2+independent calmodulin-binding protein(CaMBP)identified in Arabidopsis thaliana,which is involved in a series of important physiological activi-ties such as flower formation regulation,stomatal movement,and resistance to stress.Based on IQM gene of Arabidopsis thaliana,this study,26 IQM gene family members were identified from 6 species of Brassica,B.rapa,B.oleracea,B.nigra,B.napus,B.juncea and B.carinata by bioinformatic a-nalysis,among them 7 IQM gene family members were identified in B.oleracea,which were distributed on six chromosomes.Whole genome analysis divided 7 IQM genes of B.napus into three sub-families,and determined their genetic structure,protein conservative motif,physical location,evolutionary rela-tionships.The results showed that the genetic structure and the distribution of protein conservative motifs were relatively conservative,and gene loss occurred during the evolution.Large fragment replication e-vents happened in 4 pairs of genes as a result of natural selection.The function of BnIQM gene was also studied.The results of protein interaction network analysis indicated that some BnIQM genes may be in-volved in the response of plant to biotic and abiotic stresses.RNA-seq analysis showed that several IQM genes were highly expressed in leaves and inflorescences of B.napus L.,and different genes showed dif-ferent expression patterns in different developmental stages and tissues,indicating that functional differen-tiation of genes occurred during the process of evolution.At the same time,analysis of gene expression under abiotic stress showed that most of the IQM genes in B.napus responded obviously to several kinds of abiotic stresses.
万方数据
维普
