Evaluation of Immune Effect of Corynebacterium pseudotuberculosis Inactivated Vaccine on Goats
Corynebacterium pseudotuberculosis(Cp)is an important pathogen responsible for surface ab-scesses and caseous lymphadenitis(CLA)of goats,and vaccination is one of the most effective methods to prevent and control Cp infection.In this study,Cp Wanzhou strain(WZ)was used as the vaccine candi-date,formaldehyde as the inactivating agent,and ISA 201 VG as the adjuvant to prepare an inactivated vaccine.The goats were immunized with Cp vaccine for twice with 28 d interval,and were challenged with Cp WZ at 30th days after the second immunization.The goats were continuously observed for 65 d.The immunoprotective effect were evaluate by detecting the weight gain,temperature,routine blood,serum IgG levels,the number of lymph node abscesses and the bacterial load in the goats.The results showed that compared with the control group,the serum IgG antibodies of the immunized goats significantly increased at the 3rd and 4th weeks after the first immunization and 2-4 weeks after the 2nd immunization,and the IgG antibodies of the 2nd immunization were significantly higher than those of the 1st immunization.The temperature of goats in control was higher than that of the immuned goats within 7 days after the challenge.The weight gain of goats in control was lower than that of the immuned ones within 1-8 weeks,and the number of abscesses and Cp in the immuned goat group were significantly lower than that of the control.The above results suggested that inactivated vaccine immunization could alleviate the weight loss and the body temperature increase caused by Cp infection in goats,reduce the number of ab-scesses and Cp bacterial load in lymph node caused by Cp infection.In summary,the prepared Cp inactiva-ted vaccine could improve the immune level of goats and had a good protective effect against Cp infection.
万方数据
维普
