Evaluation of the Immunoprotective Effect of Surface Antigen SAG12 of Eimeria tenella on Chickens
To evaluate the immunoprotective effect of the surface antigen (SAG) SAG12 of Eimeria tenella (E. tenella) on chickens. The prokaryotic expression vector EtSAT12/pET-32a was constructed. The Et-SAG12 recombinant protein was purified and polyclonal antibody was prepared. In the animal experiment,100 one-day-old Hy-Line Sonia male chicks were divided into five groups:infection control group (PC),blank control group (NC),and EtSAG12 recombinant protein 50 μg,100 μg,and 150 μg groups. The im-munoprotective effect was assessed by measuring relative weight gain,oocyst reduction rate,lesion score,anti-coccidial index (ACI) and the levels of IgG in serum. The results showed that the EtSAGR12 recom-binant protein was about 43 Ku in size and mainly expressed in soluble form. Western Blot showed that the antibody had good specificity. Compared to the PC group,the three immunized groups showed signifi-cantly higher weight gain (p<0.05),lower lesion scores (p<0.05),and reduced oocyst output. In the group immunized with 150 μg,the relative weight gain (97.34％) and the oocyst reduction rate (52.54％) were the highest,with an ACI of 165.34,indicating moderate anticoccidial effects. The levels of IgG in se-rum antibody levels were significantly higher in the immunized groups than in the PC and NC groups (p<0.05),and reached peak at 7 days after the second immunization,with the 150 μg group showing the high-est levels (p<0.05). In summary,The EtSAG12 recombinant protein can reduce weight loss and oocyst shedding,stimulate humoral immunity in the host,and have a certain degree of immunoprotective effect a-gainst coccidial infection.
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