Pathogenicity and Drug Sensitivity Analysis of Proteus Mirabilis from Five Bovine Proteus mirabilis Strains
In order to explore the differences in pathogenicity and drug resistance among five bovine Pro-teus mirabilis strains,In this study,the five strains (SPL569,SPL1502,SPL1504,SPL1511,SPL1512) isolated previously were further identified by routine bacteriological analysis,swarming motility analysis,16S rDNA sequence alignment,animal pathogenicity test and virulence factor detection. Drug resistance was analyzed by drug sensitivity test and drug resistance gene detection. The results showed that the 5 strains were all Proteus mirabilis,and all had concentric circle migration on a 1.5％ agar plate. SPL1511 and 1512 migrated faster than other strains and had stronger swarming motility. The results of the patho-genicity test showed that only SPL1511 and SPL1512 were lethal to mice. Virulence gene detection showed that only SPL1511 and SPL1512 had unique rsbA gene. It not only confirmed that the migration ability regulatory factor (rsbA) gene is the key to the pathogenicity,but also verified that the stronger the swarming motility ability,the stronger the invasiveness. The results of the drug sensitivity test showed that the 5 strains were basically resistant to most common antibiotics in clinical practice,among which SPL569,SPL1502,and SPL1504 strains were only sensitive to ceftriaxone,SPL1511 was only sensitive to ceftazidim,and SPL1512 was only sensitive to cefoperazone. The results of drug resistance gene detection were also consistent with the results of drug sensitivity test in this study.
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