Exploration of Chemical Mutagenesis System and Construction of Mutant Library for Chinese Yam Bulbil
Study of ethyl methanesulfonate (EMS) chemical mutagenesis of Chinese yam bulbil can not only obtain abundant mutant materials and provide a good material basis for subsequent gene function analysis and new variety selection,but also is of great significance for enriching yam breeding techniques and methods. In this study,the sensitivity of EMS to different materials was investigated by using 0(CK),0.6％,0.8％,1.0％,and 1.2％ concentrations of EMS to treat the bulbils of Baiyu,Jin yam 1,Fenyang Jicun yam,Pingyao Yuebi yam,and Shandong Ximao yam Zero Yu Zi to screen the most sensi-tive materials for EMS. Pingyao Yuebi yam,the most sensitive to EMS,was selected for further treat-ment to investigate the phenotypic variation after mutagenesis treatment and to construct a mutant library of this material. The post-representative variation rate of the main stem was the highest,with a variable rate of 3.86％,accounting for 41.24％ of the total variable rate. The variation of leaf shape was the most abundant,with 12 variation types and a variation rate of 3.46％,accounting for 36.97％ of the total varia-tion rate. EMS at concentrations ranging from 0.81％ to 1.20％ effectively induced a wide array of pheno-typic variations in yam bulbils. This study provides valuable mutant materials for yam germplasm innova-tion and the development of new yam varieties.
万方数据
维普
