Quality Evaluation of Granati Pericarpium Dispensing Granules Based on Standard Decoctions
To establish a quality evaluation method for Granati Pericarpium formula granules based on standard decoctions,15 batches of Granati Pericarpium standard decoctions and 3 batches of dispensing granules were prepared,the extraction rates were determined. The specific chromatogram was established based on high performance liquid chromatography (HPLC),and the similarity was evaluated. The chemo-metric methods including unsupervised cluster analysis (HCA),principal component analysis (PCA) and supervised partial least squares discriminant (PLS-DA) were also used for analysis of the characteristic chromatogram data. The content of the index components were detected with HPLC,and the transfer rates of these components in standard decoctions and dispensing granules were calculated. The results showed that similarity of the specific chromatograms between standard decoctions and dispensing granules were all above 0.9. Extraction rates of 15 batches of Granati Pericarpium standard decoctions were 27.95％-41.32％. The transfer rates of punicalin,punicalagin and ellagic acid in standard decoctions were 21.49％-39.00％,18.99％-33.62％,53.37％-95.45％,respectively. The allowable concentration ranges of those components in dispensing granules were 10.58-19.64 mg/g,29.91-55.55 mg/g,10.51-19.52 mg/g,respectively. Extraction rates of dispensing granules were 41.9％-43.1％. The transfer rates of index compounds in dispensing granules were close to these in standard decoctions,and the contents of these compounds in dispensing granules was within the scope of the quality standard. In this study,the quality control standard of Granati Pericarpium formula granules was established based on the standard decoction,which provided a reference for the quality control and process research of Granati Pericarpium formula granules.
万方数据
维普
