Protective effects of macrophage migration inhibitory factor inhibitor on brain injury in rats with subarachnoid hemorrhage
Objective To investigate the protective effects of macrophage migration inhibitory factor(MIF)inhibitor on brain injury in rats with subarachnoid hemorrhage(SAH).Methods A total of 120 12-month-old healthy male rats were randomly divided into Sham surgery group,SAH group,low-dose,medium-dose and high-dose inhibitor groups according to the random number table method,with 24 rats in each group.The SAH rat animal models were created via double autologous blood injection into the cisterna magna.Rats in the low-dose,medium-dose and high-dose inhibitor groups were injected intraperitoneally with MIF inhibitor at dosages of 10 mg/kg,20 mg/kg and 40 mg/kg,respectively.Inflammatory factors,neuronal cell apoptosis,brain water content,blood-brain barrier permeability and neurologic function were determined in the five groups of rats.Results The expressions of interleukin-1β,tumor necrosis factor-α,and interleukin-6 in the brain tissues of rats,percentage of apoptotic neuronal cells,brain edema index,Evans blue content,and neurobehavioral scores of rats were compared among the five groups,and showed statistically significant differences(F=76.406,62.664,64.106,40.211,24.869,60.229,43.664;all P<0.01).Compared with the Sham surgery group,the expressions of interleukin-1β,tumor necrosis factor-α,and interleukin-6,percentage of apoptotic neuronal cells,brain edema index,and Evans blue content in the SAH group were significantly elevated,while the neurobehavioral scores were substantially decreased(P<0.01).Intraperitoneal injection of MIF inhibitor significantly reversed the aforementioned effects and demonstrated a dose-dependent relationship(all P<0.05).Conclusion The MIF inhibitor displays an obvious protective effect on brain injury in SAH rats and exhibits a dose-response relationship.
万方数据
维普
