人巨细胞病毒微小RNA UL112调控血管内皮细胞mRNA的研究
Study of human cytomegalovirus microRNA UL112 regulating vascular endothelial cell mRNA
沈凯 1邵满芬 1蔡聪慧1
作者信息
- 1. 316000 浙江省舟山市妇女儿童医院(舟山市第三人民医院)心血管内科
- 折叠
摘要
目的 探讨人巨细胞病毒RNA UL112 对人血管内皮细胞mRNA表达的影响.方法 构建人巨细胞病毒微小RNA UL112 腺病毒过表达载体并使其感染人原代脐静脉内皮细胞作为转染组,感染无义空白腺病毒载体的脐静脉内皮细胞为对照组.检测人巨细胞病毒微小RNA UL112 对脐静脉内皮细胞mRNA表达谱的影响.结果 与对照组相比,转染组mRNA存在差异表达基因365个,其中表达基因上调303个,下调62个,差异表达基因富集于丝裂原活化蛋白激酶通路.结论 人巨细胞病毒微小RNA UL112影响脐静脉内皮细胞mRNA的表达.
Abstract
Objective To investigate the effect of human cytomegalovirus microRNA UL112(HCMV miR-UL112)on human vascular endothelial.Methods A HCMV miR-UL112 adenovirus overexpression vector was constructed and used to infect human primary umbilical vein endothelial cells(HUVECs)as the transfection group,while HUVECs infected with an ineffective adenovirus served as the control group.The effect of HCMV miR-UL112 on mRNA expression profile of HUVECs were detected.Results In the transfection group,there were 365 differentially expressed genes compared to the control group,with 303 upregulated genes and 62 downregulated genes.The differentially expressed genes were enriched in the mitogen-activated protein kinase(MAPK)signaling pathway.Conclusion HCMV miR-UL112 can affect mRNA expression of HUVECs.
关键词
人巨细胞病毒微小RNA/UL112/差异表达/丝裂原活化蛋白激酶通路Key words
Human cytomegalovirus microRNA-UL112/Differential expressiom/Mitogen-activated protein kinasesignaling pathway引用本文复制引用
基金项目
浙江省自然科学基金(2013LY13H020001)
舟山市科技计划(2012C13024)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据