Early observation of atrophy and miRNA-206 expression changes in hind limb skeletal muscles of rats with tethered cord syndrome
Objective To observe early pathological changes of skeletal muscle atrophy in posterior tibial muscle group of hind limbs by establishing a rat model of tethered cord syndrome(TCS),to explore the changes of miRNA-206 in this process and to examine its effect on skeletal muscle atrophy.Methods The TCS rat model was prepared surgically.The study observed early functional impairment and changes in muscle volume,wet muscle weight(ratio),as well as average cross-sectional area of muscle fibers in posterior tibial muscle group of hind limbs after modeling and antagonist dosing.Real-time fluorescent quantitative polymerase chain reaction was employed for detecting the expression of miRNA-206 in hind limbs.Results As of Day 7 postoperatively,functional impairment appeared in hind limbs of model group.At Day 28,the expression of miRNA-206 spiked markedly in model group.It was 5.54 folds that of sham operation group.Wet weight and average cross-sectional area of muscle fiber declined obviously.As compared with sham operation Day 28 group,the difference was statistically significant(all P＜0.05).After dosing of miRNA-206 antagonist,average cross-sectional area of muscle fiber,volume of posterior tibial muscle group and muscle wet weight ratio declined greatly.And the differences were statistically significant(P＜0.05).Conclusion In skeletal muscle atrophy of posterior tibial muscle groups in hind limbs of TCS rats,early pathological changes lead to a marked up-regulation of miRNA-206.This may represent a form of self-protection mechanism by the organism.
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