Objective To study the peripheral blood immunogram changes during early life in premature infants with bronchopulmonary dysplasia(BPD).Methods From April 2022 to June 2022,premature infants with gestational age ≤32 weeks admitted to our hospitals were enrolled.The infants were assigned into BPD group and non-BPD group according to BPD diagnostic criteria 2001 edition.Peripheral blood(1 ml)was collected 28 d after birth and within 36 weeks of corrected gestational age and examined using 17-color flow cytometry.Peripheral blood immunograms were compared between the two groups.Results A total of 14 infants were enrolled,including 9 in BPD group and 5 in non-BPD group.BPD group showed significantly lower percentage of both adaptive immune cells and innate immune cells than non-BPD group,adaptive immune cells:CD4+naive T cells[7.9％(1.2％,10.0％)vs.24.8％(15.8％,29.7％)],plasmablast cells[0.01％(0.00％,0.07％)vs.0.14％(0.04％,0.18％)];innate immune cells:neutrophils[0.66％(0.52％,1.58％)vs.3.94％(2.29％,6.02％)],CD127+polymorphonuclear myeloid-derived suppressor cells(PMN-MDSCs)[13.4％(4.3％,59.8％)vs.74.8％(58.2％,83.3％)]and CD127+monocytic(M)-MDSCs[3.6％(3.1％,5.5％)vs.22.0％(8.3％,22.5％)](all P＜0.05).Conclusions The decrease of neutrophils,CD127+PMN-MDSCs and other immune cells during early life in premature infants may contribute to the sustained inflammatory responses and pathogenesis of BPD.
维普
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