Association of HPA gene polymorphism and interaction with neonatal thrombocytopenia
Objective To explore the interaction between the polymorphism of human platelet antigens(HPA)and neonatal thrombocytopenia(NT),and to provide experimental basis for the study of the pathogenesis of NT.Methods A total of 105 patients with NT who were hospitalized in the Department of Neonatology(from January 2022 to October 2023)were included in the NT group,and 124 children with undiagnosed NT were included in the control group.Peripheral blood was collected and genotyping of HPA was performed via amplification refractory mutation system PCR(ARMS-PCR)and the specificity of the amplified products was analyzed by melting curve detection analysis with real time quantitative PCR.Generalized Multifactor Dimensionality Reduction(GMDR)software GMDR 0.7 was used to assess the risk of gene-gene interactions in NT for HPA systems.Results There were significant differences in pregnancy complication incidence,neonatal asphyxia incidence and anti-platelet antibody positive rate between NT group and control group(P<0.05).There was no statistically significant difference in gender,birth weight,gestational age,adverse pregnancy history,pulmonary infection,neonatal septicemia,hemorrhage,coagulation abnormality and neonatal jaundice between the two groups(P>0.05).There was no significant difference in the frequency distribution of HPA1-6,15 pairs of alleles between NT group and control group(all P>0.05).Among the HPA polymorphism 1-7 interaction models,the second-order(HPA-3,15)model had the highest testing balance accuracy(0.7821)and the best CV consistency(10/10),which was statistically significant(P=0.001).The third and fourth order models were second only to the second-order(HPA-3,15),with a balanced test accuracy of 0.7695 and a cross validation consistency of 9/10,which was statistically significant(P<0.05).Conclusion The second,third and fourth order models were related to the occurrence of NT,and the second order model was the best model,suggesting a potential interaction between HPA-3 and HPA-15 in the occurrence of NT.
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