Experimental study of slow blood flow model in rats
Objective After repeated subcutaneous injection of epinephrine and swimming in ice water,the hemorheology indexes and ultrasound results of both lower limbs were observed to construct the slow blood flow model of rats before thrombosis.Methods Twelve healthy SD rats were selected,the epinephrine group(n=6)was injected subcutaneously with 0.8 mL/kg epinephrine,swam in ice water for 5 minutes 2 hours later,injected again 4 hours later,and fasted for 16 hours.The normal saline group(n=6)was injected subcutaneously with 0.8 mL/kg normal saline,and the preparation method was consistent with that of epinephrine group.After the molding was completed,the femoral veins of both lower limbs were examined by ultrasonography and hemorheology test.Result There was no significant swelling in the lower limbs of rats in both groups.After swimming in ice water for 1 min,rats in epinephrine group were more excited than those in normal saline group(P=0.015).Ultrasound results showed that there was no obvious thrombosis in the femoral vein in both groups,but the right femoral vein flow rate in the adrenalin group was lower than that in the normal saline group(P<0.001).The hemorheological results showed that the whole blood viscosity was lower(P=0.024)and the plasma viscosity was higher(P=0.004)in epinephrine group than in normal saline group.Conclusion By repeatedly injecting epinephrine subcutaneously and swimming in ice water,and by using hemorheology and ultrasound measurement,the rat model of slow blood flow can be constructed.
slow blood flowadrenalinswimming in ice waterdeep vein thrombosis
NETL
NSTL
万方数据
