Effect and Mechanism of Galangal Diphenylheptane Compound on 3T3-L1 Preadipocyte Differentiation
Objective To investigate the effect and mechanism of galangal diphenylheptane compound on 3T3-L1 pre-adipocyte differentiation.Methods This study selected 3T3-L1 preadipocytes from Zhuhai People's Hospital and Ji-nan University Affiliated Zhuhai Hospital from September 2022 to February 2023 as the research subjects,and used the MTT method to evaluate the effect of galangal diphenylheptane compounds on the proliferation of 3T3-L1 cells.The effect of Alpinia officinalis diphenylheptane compounds on 3T3-L1 cell differentiation was analyzed through O il Red O staining.The regulatory effect of Galangal diphenylheptane compounds on mRNA expression of peroxisome proliferator activated receptor γ(PPARγ)and CCAAT/enhancer binding protein α(C/EBPα)were detected through polymerase chain reaction(PCR)method.The effect of Galangal diphenylheptane compounds on the protein expres-sion of PPARγ and C/EBPαthrough Western blot analysis.Results Galangal diphenylheptyl compounds(0,0.05,0.2,0.4 g/L)showed a proliferation trend on the growth of preadipocytes at 24 h and 48 h,showing a certain dose-effect re-lationship.When the concentration was 0.4 g/L,the difference was statistically significant compared with the control group(7.02±0.35)g/L and(17.23±0.95)g/L(t=13.794,10.843,both P<0.05).Galangal diphenylheptane compounds inhibited the proliferation of 3T3-L1 cells in a dose-dependent manner.The results of oil red O staining showed that the diphenylheptane compound of galangal significantly inhibited the adipocyte differentiation of 3T3-L1 cells and re-duced the accumulation of lipid droplets.mRNA expression levels of PPARγ and C/EBPα were significantly de-creased in the 100µmol/L treatment group.Galangal diphenylheptane compounds regulate the differentiation of 3T3-L1 preadipocytes by inhibiting the expression of PPARγ and C/EBPα genes,thereby reducing the accumulation of fat droplets.Conclusion Galangal diphenylheptane compounds inhibit 3T3-L1 preadipocyte differentiation,and the mechanism is related to the low expression of PPARr/EBPα.
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