Diagnostic Value of Fluorescence Quantitative PCR and Immunocolloidal Gold in Monitoring Infection in Patients undergoing Osteoporosis Surgery
Objective To investigate the clinical effect of quantitative polymerase chain reaction(PCR)and immuno-colloidal gold detection on infection monitoring of inpatients undergoing operative treatment for osteoporosis.Methods A total of 180 patients with osteoporosis undergoing surgical treatment and suspected inpatient infection in the First People's Hospital of Qujing City from March 2020 to April 2023 were selected as the study objects.All patients were detected by fluorescence quantitative PCR and immunocolloidal gold method successively,and the detection rates of the two methods were compared using the results of bacterial culture as the gold standard.Evaluate the diagnostic effi-cacy of the two inspection methods.Results The results of bacterial culture were positive in 37 cases,fluorescent quantitative PCR in 35 cases,and immunocolloidal gold in 26 cases.The accuracy,sensitivity,specificity,positive predictive value and negative predictive value of fluorescence quantitative PCR were 96.67%,89.19%,98.60%,94.29%,97.24%,and 82.78%,43.24%,93.01%,61.54%,86.36%for immunocolloidal gold method,respectively.The accuracy,sensitivity,specificity,positive predictive value and negative predictive value of fluorescence quantitative PCR were significantly higher than those of immunocolloidal gold,and the differences were statistically significant(χ2 =18.827,17.458,5.567,10.124,11.533,all P<0.05).Conclusion Fluorescence quantitative PCR assay has a good di-agnostic efficacy in monitoring infection in inpatients undergoing operative treatment for osteoporosis.The intervention of microbiological test in infection monitoring of orthopedic inpatients is conducive to early diagnosis and treatment based on the test results,thus reducing the incidence of infection and the risk of transmission.
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