Objective To explore the role of lyso-PL acyltransferases 3(LPCAT3)in oscillatory flow(OSS)mediated endothelial cell activation.Methods Aortic arch(AA)and thoracic aorta(TA)of 5 C57BL/6 male mice were isolated from Jining First People's Hospital from November 2020 to June 2023 for mRNA extraction and detection of LPCAT3 expression.The primary endothelial cells were randomly divided into control group(Static)and study group(OSS)to detect the mRNA level of LPCAT3.At the same time,LPCAT3 was knocked down to verify the mRNA expression of vascular cell adhesion molecule 1(VCAM-1)and intercellular adhesion molecule 1(ICAM-1).Results Compared with thoracic aorta(1±0.42),the mRNA relative expression level of LPCAT3 in mouse aortic arch was significantly up-regulated(2.03±0.5),and the difference was statistically significant(P＜0.05).The mRNA expression level of LPCAT3 in endothelial cells of the study group was 1.88 times higher than that of the control group,and the difference was sta-tistically significant(P＜0.05).Compared with the mRNA relative expression level of the study group(2.72±0.72)(3.04±0.56),the expression of VCAM-1 and ICAM-1 in OSS+siLPCAT3 group was significantly decreased 1.31±0.4)(1.81±0.51),and the differences were statistically significant(both P＜0.05).This implied that inhibition of endothelial LPCAT3 can significantly reduce the expression of turbulence-mediated inflammatory factors.Conclusion LPCAT3 regulates turbulence-mediated endothelial cell activation.
万方数据
维普
