Effect of Brucea Javanica Combined with Pirarubicin on Bladder Cancer Cells
Objective To explore the effect of brucea javanica combined with pirarubicin on bladder cancer cells.Methods A human BIU-87 bladder cancer cell line from Department of Urology of Heilongjiang Provincial Hospital during April to May 2023 was selected as the research object.After cell culture,the cell lines were inoculated into 96-well plates and divided into 5 groups by random number table method.Three parallel samples were taken from each group of cells,and five multiple pores were set in each sample(15 samples in total for each group).Five groups of cells were treated with different concentrations of brucea javanica oil emulsion(0,5,10,20,30 mL/L)combined with pi-rarubicin(5 µmol/L).The effects of different concentrations of brucea javanica oil emulsion on proliferation inhibition,apoptosis and cell cycle of bladder cancer cells were analyzed.The effects of combined administration on nuclear fac-tor κB(NF-κB)and the expression of adenosine triphosphate(ATP)combined with ATP-binding cassette transpor-erG2(ABCG2)in cells were detected.Results The proliferation inhibition rate,necrosis rate and apoptosis rate of BIU-87 cells were increased with the increase of brucea javanica oil emulsion concentration,the difference was statisti-cally significant(all P＜0.05).The cell proliferation inhibition rate and cell necrosis rate were significantly increased when the concentration of brucea javanica oil was 5 mL/L,and the apoptosis rate was significantly increased when the concentration was greater than 10 mL,the difference was statistically significant(all P＜0.05).The proportion of S phase cells in BIU-87 cells decreased and the proportion of C0/C1 phase cells increased with the increase of the con-centration of brucea javanica oil emulsion,the difference was statistically significant(all P＜0.05).As the concentration of brucea javanica oil emulsion increases,in the cytoplasm the expression level of NF-κB gradually decreases,in the nucleus the expression level of NF-κB gradually increased,while the expression level of ABCG2 on the cell mem-brane surface decreased,the difference was statistically significant(all P＜0.05).Conclusion Brucea javanica oil emul-sion combined with pirarubicin can inhibit the proliferation of BIU-87 bladder cell line,low concentration can pro-mote cell necrosis,high concentration can promote cell apoptosis,and promote the transfer of NF-κB from cytoplasm to nucleus,and inhibit the expression of ABCG2 in a dose-dependent manner.
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维普
