Study of ELISA Combined with Fluorescent Quantitative PCR for Detec-tion of Hepatitis C Virus
Objective To evaluate the application value of enzyme-linked immunosorbent assay(ELISA)combined with fluorescence quantitative polymerase chain reaction(PCR)for hepatitis C virus detection.Methods 90 suspected hepatitis C virus patients treated by Tengzhou Center for Disease Control and Prevention from August 2022 to August 2023 were selected as the research objects.ELISA combined with fluorescence quantitative PCR test was used,and hepatitis virus C(HCV)RNA test results were taken as the"gold standard"to analyze the application value of the de-tection method.Results The HCV RNA test results showed that the positive rate was 47.78%,the positive rate of the ELISA test was 40.00%,the Quantitative fluorescence PCR found a 42.22%positive rate,the positive rate of the com-bined test was 46.67%.The differences between ELISA and quantitative PCR in sensitivity,specificity,accuracy,posi-tive and negative predictive value were not statistically significant(all P＞0.05).The sensitivity of the combined test was 93.02%,with an accuracy of 94.44%,a positive predictive value of 95.24%and a negative predictor of 93.75%,which were significantly higher than the 67.44%,76.67%,80.56%,74.07%detected by ELISA;the sensitivity and ac-curacy of the combined test were significantly higher than that of the fluorescence quantitative PCR test(76.74%,83.33%),and the differences were statistically significant(all P＜0.05).Conclusion The application of ELISA com-bined with fluorescence quantitative PCR for the detection of hepatitis C virus has a high detection rate.
万方数据
维普
