首页|胶体金法及酶联免疫法应用于艾滋病抗体检测的价值分析

胶体金法及酶联免疫法应用于艾滋病抗体检测的价值分析

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目的 分析在艾滋病病毒抗体检测中,应用酶联免疫吸附试验(Enzyme-linked Immunosorbent Assay,ELISA)和胶体金法的检验效果.方法 选取2018年6月—2023年6月海安市疾病预防控制中心复检人类免疫缺陷病毒抗体检测的90份样本作为研究对象,均应用胶体金法及ELISA法检测.比较两种检测方式的诊断效能.结果 胶体金法检测灵敏度93.33%、特异度86.67%、准确度92.22%;ELISA法检测灵敏度100.00%、特异度93.33%、准确率98.89%.ELISA法检测灵敏度及准确度均优于胶体金法,差异有统计学意义(χ2= 5.172、4.709,P均<0.05).结论 在艾滋病诊断中,应用ELISA及胶体金法均有较高的诊断效能,ELISA法检测灵敏度及准确度均优于胶体金法.
Analysis of the Value of Colloidal Gold Method and Enzyme-linked Immu-nosorbent Application for HIV Antibody Detection
Objective To analyze the test effect of application using enzyme-linked immunosorbent assay(ELISA)and colloidal gold method in antibody of human immunodeficiency virus(HIV)detection.Methods From June 2018 to June 2023,90 samples of HIV antibody retested by the Center for Disease Control and Prevention of Haian City were selected as the research objects,all of which were detected by colloidal gold method and ELISA method.The diagnos-tic efficiency of the two detection methods was compared.Results The sensitivity,specifity and accuracy of colloidal gold method were 93.33%,86.67%and 92.22%,respectively.The sensitivity,specifity and accuracy of the ELISA method were 100.00%,93.33%and 98.89%,respectively.The detection sensitivity and accuracy of ELISA method were better than those of colloidal gold method,and the differences were statistically significant(χ2=5.172,4.709,both P<0.05).Conclusion In the diagnosis of acquired immune deficiency sgndrome,the application of ELISA and colloidal gold method both have high diagnostic efficacy,and the detection sensitivity and accuracy of ELISA method are better than colloidal gold method.

AIDSELISAColloidal goldAntibody detectionDiagnostic efficacy

崔一峰

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海安市疾病预防控制中心微生物检验科,江苏南通 226600

艾滋病 酶联免疫法 胶体金法 抗体检测 诊断效能

2024

系统医学

系统医学

ISSN:
年,卷(期):2024.9(3)
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