Exploration of the Role of Hh Signaling Pathway Molecule TPX2 on the Proliferation of Oral Squamous Carcinoma
Objective To explore and analyze the role of Hedgehog(Hh)signaling pathway molecule TPX2 on the pro-liferation of oral squamous carcinoma.Methods Six lines of human oral squamous carcinoma cell line CAL-27 cul-tured in the Oral Teaching and Research Department of Hulunbeier Vocational Technical College from January 2022 to July 2023 were selected as the study objects.Three lines of human oral squamous carcinoma cell line CAL-27 transfected with Lv-pc3.0 vector were set as the control group.Three lines of human oral squamous carcinoma cell line CAL-27 transfected with Lv-pc3.0-shTPX2 were set as the Lv-shTPX2 group.Cell cycle,cell proliferation with target gene and protein expression between the two groups of human oral squamous carcinoma cell line CAL-27 were com-pared.Results At 24 h,48h after transfection,the levels of TPX2 mRNA[(5.44±0.17),(5.18±0.47)]and protein rela-tive expression[(2.01±0.23),(1.33±0.16)]in the Lv-shTPX2 group were lower than those in the control group with the levels of TPX2 mRNA[(24.59±0.33),(24.58±2.76)],and protein relative expression[(10.47±0.16),(7.48±0.33)],the differences were statistically significant(t=89.352,12.002,52.299,29.045,all P<0.05).And the cell proliferation in-dex was lower in the Lv-shTPX2 group,the difference was statistically significant(P<0.05).After 48 h of transfection,the relative proportion of G2+M-phase cell in the Lv-shTPX2 group was higher than that in the control group,the rela-tive proportion of S-phase cells was lower than that in the control group,and the relative expression levels of GLI2 and FOXM1 proteins were lower than those of the control group,the differences were statistically significant(all P<0.05).Conclusion Inhibition of the expression of Hh signaling pathway molecule TPX2 can inhibit the proliferation of oral squamous cell carcinoma cells,and also inhibit the expression of GLI2 and FOXM1 proteins,as well as regulate the cell cycle status,which may provide a new way for the treatment of oral squamous cell carcinoma.
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