Study of the Function and Mechanism of miR-324-3p/MAGOH Molecular Axis in Brain Glioma
Objective To investigate the function and mechanism of miR-324-3p/MAGOH molecular axis in brain glioma.Methods Samples of glioma patients(n=50)and non-glioma encephalopathy patients(n=20)who received sur-gical treatment in the Department of Neurosurgery of Affiliated Hospital of Zunyi Medical University from June 2022 to June 2023 were collected,and human glioma cell line U87 was cultured in vitro.the real-time quantitative PCR de-tecting system(qPCR)detects the expression of miR-324-3p and MAGOH in glioma cells(U87)and clinical speci-mens.Western blot(WB)was used to detect the expression of MAGOH,matrix metalloproteinase(MMP)2 and MMP9.Cell counting kit-8(CCK-8)is used to detect the proliferation of glioma cells.The invasive ability of glioma cells was measured by Transwell.Luciferase reporter gene to detect the interaction between miR-324-3p and MAGOH.The ex-pression of MAGOH in brain glioma was detected by immunohistochemistry.Results The expression of MAGOH in cancer tissues of glioma patients was higher,and the difference was statistically significant(P<0.01).There was a sig-nificant negative correlation with the expression of miR-324-3p(r=0.7383,P<0.001).Overexpression of MAGOH could promote the proliferation and invasion of glioma cells(P<0.01),while overexpression of miR-324-3p could in-hibit the proliferation and invasion of glioma cells(P<0.01).miR-324-3p interacts with the 3'-UTR of MAGOH,and overexpression of MAGOH can inhibit the inhibitory effect of miR-324-3p on glioma cells(P<0.01).Conclusion miR-324-3p inhibited glioma cells and proliferation and invasion by suppressing the expression of MAGOH.
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